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Abstract

A novel Gram-stain-negative, curved rod-shaped, motile and non-endospore-forming strain, designated HX-2-15, was isolated from activated sludge of agricultural chemical plant in Nanjing, Jiangsu province, PR China (32° 03′ N, 118° 46′ E) . Growth was observed at 15–37 °C (optimum between 25 and 30 °C), at pH 6.0–8.0 (optimum at pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum at 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed closest affiliation to SAY3, with a sequence similarity of 99.0 %. The predominant cellular fatty acids were C, C cyclo and summed feature 3 (C 7 and/or C 6). The major quinone was ubiquinone Q-8 . The polar lipid profile was composed of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified lipid and one unidentified aminophosphoglycolipid . The genomic DNA G+C content of the strain was 63.6 mol%. The ANI and dDDH values obtained between the genomes of HX-2-15 and SAY3 were 85.3 and 29.3 % respectively. On the basis of data from phenotypic, chemotaxonomic and genotypic analysis, strain HX-2-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HX-2-15 (=KCTC 72286=CCTCC AB 2019178).

Funding
This study was supported by the:
  • Jian He , National Science and Technology Major Project , (Award 2018ZX0800907B-002)
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/content/journal/ijsem/10.1099/ijsem.0.003762
2019-10-16
2020-09-24
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