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Volume 70, Issue 1

sp. nov., a phototrophic bacterium Free

Abstract

An oval- to rod-shaped, motile, Gram-stain-negative, oxidase-positive, catalase-negative, pink-coloured phototrophic bacterium (designated as strain JA968) was isolated from an estuary near Pata, Gujarat, India. Cells had an intracytoplasmic membrane architecture as lamellae and divided by budding. Strain JA968 had bacteriochlorophyll- and spirilloxanthin series carotenoids as photosynthetic pigments. The strain exhibited photolithoautotrophic, photoorganoheterotrophic and chemoorganoheterotrophic growth modes and required thiamine as a growth factor. Strain JA968 had Cω7/C ω6 as the predominant fatty acid with ubiquinone-10 (Q-10) and menaquinone-10 (MK-10) forming the quinone composition. The genomic DNA G+C content of the strain was 63.5 mol%. Pairwise comparison of 16S rRNA gene sequences showed that strain JA968 was highly similar to DSM 2698 (99.9 %) and DSM 17143 (98.4 %). The average nucleotide identity values were 92 % between strain JA968 and DSM 2698, and 78 % between strain JA968 and DSM 17143. The digital DNA–DNA hybridization values between strain JA968 and and were 49 and 19 %, respectively. The genomic distinction was also supported by differences in phenotypic and chemotaxonomic characteristics. We propose that strain JA968 represents a new species of the genus with the name sp. nov. The type strain is JA968 (=KCTC 15634=NBRC 113338).

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  • Accepted:
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Keyword(s): Afifella , alphaproteobacteria and photosynthetic
© 2020 The Authors
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2019-10-08
2024-04-26
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References

  1. Urdiain M, López-López A, Gonzalo C, Busse H-J, Langer S et al. Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . Syst Appl Microbiol 2008; 31:339–351 [View Article]
     [Google Scholar]
  2. Imhoff JF. Rhodopseudomonas marina sp. nov., a new marine phototropic purple bacterium. Syst Appl Microbiol 1983; 4:512–521 [View Article]
     [Google Scholar]
  3. Hiraishi A, Urata K, Satoh T. A new genus of marine budding phototrophic bacteria, Rhodobium gen. nov., which includes Rhodobium orientis sp. nov. and Rhodobium marinum comb. nov. Int J Syst Bacteriol 1995; 45:226–234 [View Article]
     [Google Scholar]
  4. Caumette P, Guyoneaud R, Duran R, Cravo-Laureau C, Matheron R. Rhodobium pfennigii sp. nov., a phototrophic purple non-sulfur bacterium with unusual bacteriochlorophyll a antennae, isolated from a brackish microbial mat on Rangiroa atoll, French Polynesia. Int J Syst Evol Microbiol 2007; 57:1250–1255 [View Article]
     [Google Scholar]
  5. Lakshmi KVNS, Sasikala C, Takaichi S, Ramana CV, Ch S. Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 2011; 61:1656–1661 [View Article]
     [Google Scholar]
  6. Divyasree B, Suresh G, Sasikala C, Ramana CV. Chryseobacterium salipaludis sp. nov., isolated at a wild ass sanctuary. Int J Syst Evol Microbiol 2018; 68:542–546 [View Article]
     [Google Scholar]
  7. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article]
     [Google Scholar]
  9. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  10. López-Hermoso C, de la Haba RR, Sánchez-Porro C, Papke RT, Ventosa A. Assessment of MultiLocus sequence analysis as a valuable tool for the classification of the genus Salinivibrio . Front Microbiol 2017; 8:1107 [View Article]
     [Google Scholar]
  11. Liu Y, Lai Q, Göker M, Meier-Kolthoff JP, Wang M et al. Genomic insights into the taxonomic status of the Bacillus cereus group. Sci Rep 2015; 5:14082 [View Article]
     [Google Scholar]
  12. Rodriguez-R LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. Peer J Preprints 4e1900v1
     [Google Scholar]
  13. Auch AF, Klenk H-P, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2010; 2:142–148 [View Article]
     [Google Scholar]
  14. Rosselló-Móra R, Amann R. Past and future species definitions for bacteria and archaea. Syst Appl Microbiol 2015; 38:209–216 [View Article]
     [Google Scholar]
  15. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article]
     [Google Scholar]
  16. Wattam AR, Davis JJ, Assaf R, Boisvert S, Brettin T et al. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 2017; 45:D535–D542 [View Article]
     [Google Scholar]
  17. Choudhary M, Zanhua X, Fu YX, Kaplan S. Genome analyses of three strains of Rhodobacter sphaeroides: evidence of rapid evolution of chromosome II. J Bacteriol 2007; 189:1914–1921 [View Article]
     [Google Scholar]
  18. Groth AC, Calos MP. Phage integrases: biology and applications. J Mol Biol 2004; 335:667–678 [View Article]
     [Google Scholar]
  19. Hanada S, Takaichi S, Matsuura K, Nakamura K. Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 2002; 52:187–193 [View Article]
     [Google Scholar]
  20. Divyasree B, Suresh G, Sasikala C, Ramana CV. Chryseobacterium salipaludis sp. nov., isolated at a wild ass sanctuary. Int J Syst Evol Microbiol 2018; 68:542–546 [View Article]
     [Google Scholar]
  21. Trüper HG, Pfennig N. Characterization and identification of the anoxygenic phototrophic bacteria. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG. (editors) The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria 1 New York: Springer; 1981 pp 298–312
     [Google Scholar]
  22. Ramaprasad EVV, Sasikala C, Ramana CV, Ch S, Ch.V R. Neurosporene is the major carotenoid accumulated by Rhodobacter viridis JA737. Biotechnol Lett 2013; 35:1093–1097 [View Article]
     [Google Scholar]
  23. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical note 101. Newark, DE: MIDI Inc; 1990
  24. Kates M. Techniques of lipidology. Isolation, analysis and identification of lipids. In Burdon RH, van Knippenberg PH. (editors) Laboratory Techniques in Biochemistry and Molecular Biology 3 Amsterdam: Elsevier; 1986
     [Google Scholar]
  25. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  26. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
     [Google Scholar]
  27. Oren A, Duker S, Ritter S. The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 1996; 138:135–140 [View Article]
     [Google Scholar]
  28. Kates M. Techniques in lip idology. Laboratory Techniques in Biochemistry and Molecular Biology 2 American Elsevier Publishing Company 3; 1972 pp 355–356
     [Google Scholar]
  29. Imhoff JF. Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 1984; 25:85–89 [View Article]
     [Google Scholar]
  30. Hiraishi A, Hoshino Y. Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 1984; 30:435–448 [View Article]
     [Google Scholar]
  31. Hiraishi A, Hoshino Y, Kitamura H. Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 1984; 30:197–210 [View Article]
     [Google Scholar]
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Afifella aestuarii sp. nov., a phototrophic bacterium
Int J Syst Evol Microbiol 70, 327 (2020); https://doi.org/10.1099/ijsem.0.003756
/content/journal/ijsem/10.1099/ijsem.0.003756
/content/journal/ijsem/10.1099/ijsem.0.003756
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