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Volume 70, Issue 1

sp. nov., isolated from Weddell seals in Antarctica Free

Abstract

A taxonomic study was carried out on four Gram-stain-negative strains P5773, P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus and found and to be their closest neighbours with 99.9 and 99.2 % sequence similarity, respectively. Sequence analysis of , and housekeeping genes confirmed the highest similarity of isolates to () and to ( and ). The average nucleotide identity value below 86 %, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773 to its phylogenetic neighbours. The complete genome of strain P5773 was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C 7), summed feature 3 (C /C 6) and C. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining species and confirmed that they represent a novel species within the genus , for which the name sp. nov. is suggested. The type strain is P5773 (=CCM 8849=LMG 30618).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Antarctica , polyphasic taxonomy , Pseudomonas leptonychotis and Weddell seal
Funding
This study was supported by the:
  • Masarykova Univerzita (Award MUNI/A/0958/2018)
    • Principle Award Recipient: Roman Pantůček
  • Ministerstvo Zdravotnictví Ceské Republiky (Award 16-29916A)
    • Principle Award Recipient: Roman Pantůček
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award CZ.02.1.01/0.0/0.0/16_013/0001708)
    • Principle Award Recipient: Ivo Sedláček
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award LM2015078)
    • Principle Award Recipient: Ivo Sedláček
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award LM2015043)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2024-05-05
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Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica
Int J Syst Evol Microbiol 70, 302 (2020); https://doi.org/10.1099/ijsem.0.003753
/content/journal/ijsem/10.1099/ijsem.0.003753
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