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Volume 70, Issue 1

sp. nov., a bacterium isolated from marine sponge Free

Abstract

A taxonomic study was carried out on strain HN-E21, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E21 were Gram-stain-negative, non-spore-forming, tumbling-motile, ovoid- to rod-shaped and pale-yellow-pigmented that could grow at 15–42 °C (optimum, 37 °C), at pH 6–11 (pH 7) and in 0–14 % (w/v) NaCl (2–3 %). This isolate was positive for oxidase and catalase, but negative for hydrolysis of aesculin and gelatin. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HN-E21 formed a clade with BH-SD19, GRP21, M-S13-148, CAU1059 and NH52J within the family . Strain HN-E21 shared the highest similarity to BH-SD19 (95.5 %), followed by 112 (95.3 %), D1-W8 (95.2 %), YM-20 (95.0 %) and H50 (95.0 %). The dominant fatty acids were summed feature 8 (Cω7 and/or Cω6) and C The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified lipids and one unidentified phospholipid. The respiratory quinone was identified as Q-10. The G+C content of the genomic DNA was 66.4 mol%. Based on the phenotypic and phylogenetic data, strain HN-E21 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E21 (=MCCC 1K03333=LMG 30456).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): marine sponge , Roseovarius spongiae sp. nov. and taxonomy
Funding
This study was supported by the:
  • the Scientific Research Project of Xiamen Southern Ocean Center (Award No.17GYY008NF04)
    • Principle Award Recipient: Lianzhong Luo
  • the Young and the Middle-Aged Teacher Education Scientific Research Project of Fujian Province, China (Award No. JT180644)
    • Principle Award Recipient: Lingping Zhuang
  • the Funds for Creative Research Groups of Fujian Colleges and Universities (Award NO. MJK2018-49)
© 2020 The Authors
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2020-02-03
2024-05-12
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Roseovarius spongiae sp. nov., a bacterium isolated from marine sponge
Int J Syst Evol Microbiol 70, 274 (2020); https://doi.org/10.1099/ijsem.0.003750
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