1887

Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated T58, was isolated from a marine sediment sample collected from the coastal area of Weihai, PR China. Strain T58 was most closely related to SW033 with 97.1 % 16S rRNA gene sequence similarity, followed by KCTC 22242 (96.9 %). Based on 16S rRNA gene sequences, the phylogenetic analysis indicated that strain T58 represented a member of the genus within the family of the phylum . Strain T58 was found to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. The major fatty acids were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The major isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content of the genomic DNA was 35.0 mol%. On the basis of its phylogenetic, phenotypic and chemotaxonomic characteristics, strain T58 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T58 (=KCTC 72081=MCCC 1H00359).

Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • the National Natural Science Foundation of China (Award 31770002)
    • Principle Award Recipient: Zong-Jun Du
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2020-02-03
2024-12-04
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References

  1. Yoon BJ, Lee DH, Kang BJ, Kahng HY, Oh YS et al. Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 2010; 60:382–386 [View Article]
    [Google Scholar]
  2. Park SC, Choe HN, Hwang YM, Baik KS, Kim SN et al. Marinivirga aestuarii gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from marine environments, and emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and the species Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans. Int J Syst Evol Microbiol 2013; 63:1524–1531 [View Article]
    [Google Scholar]
  3. Shi MJ, Xie ZH, Zhang H, Du ZJ. Hyunsoonleella rubra sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2017; 67:386–390
    [Google Scholar]
  4. Gao X, Zhang ZH, Dai XF, Zhang XH. Hyunsoonleella pacifica sp. nov., isolated from seawater of South Pacific Gyre. Int J Syst Evol Microbiol 2015; 65:1155–1159 [View Article]
    [Google Scholar]
  5. Kim H, Lee JB, Oh HW, Cha HK, Yang JH et al. Hyunsoonleella udoensis sp. nov., isolated from a gravel sample from a beach of Udo island, Korea. Antonie van Leeuwenhoek 2015; 108:1009–1014 [View Article]
    [Google Scholar]
  6. Oren A, Garrity GM. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 2016; 66:1–3 [View Article]
    [Google Scholar]
  7. Mu DS, Liang QY, Wang XM, Du ZJ, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:230
    [Google Scholar]
  8. Jin S, Xia J, Dunlap CA, Rooney AP, Du ZJ. Psychroflexus saliphilus sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2016; 66:5124–5128
    [Google Scholar]
  9. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article]
    [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  11. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
    [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  14. RQ Li, Zhu HM, Ruan J, Qian WB, Fang XD et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272
    [Google Scholar]
  15. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
    [Google Scholar]
  16. Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196:2210–2215 [View Article]
    [Google Scholar]
  17. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  18. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  19. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070
    [Google Scholar]
  20. Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M et al. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense. Int J Syst Evol Microbiol 2007; 57:1095–1107 [View Article]
    [Google Scholar]
  21. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article]
    [Google Scholar]
  22. Dong XZ, Cai MY. Determination of biochemical characteristics. In Dong XZ, Cai MY. (editors) Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp 370–398
    [Google Scholar]
  23. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696
    [Google Scholar]
  24. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42:457–469 [View Article]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
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