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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 1

sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium Free

Abstract

Thermotolerant bacterial nanocellulose-producing strains, designated MSKU 9 and MSKU 15, were isolated from persimmon and sapodilla fruits, respectively. These strains were aerobic, Gram-stain-negative, had rod-shaped cells, were non-motile and formed white–cream colonies. Phylogeny based on the 16S rRNA gene sequences revealed that MSKU 9 and MSKU 15 represented members of the genus and formed a monophyletic branch with JCM 17123 and DSM 6160. The genomic analysis revealed that overall genomic relatedness index values of MSKU 9 with JCM 17123 and DSM 6160 were ~90 % average nucleotide identity (ANI) and ≤58.2 % digital DNA–DNA hybridization (dDDH), respectively. MSKU 9 and MSKU 15 can be differentiated from the closely related JCM 17123 by their growth on 30 % -glucose and ability to utilize and to form acid from raffinose and sucrose as carbon sources, and from DSM 6160 by their ability to grow without acetic acid. The genomic DNA G+C contents of MSKU 9 and MSKU 15 were 60.4 and 60.2 mol%, respectively. The major fatty acids of MSKU 9 and MSKU 15 were summed feature 8 (C ω7c and/or Cω6c). The respiratory quinone was determined to be Q10. On the basis of the results of the polyphasic taxonomic analysis, MSKU 9 (=TBRC 9844=NBRC 113802) represents a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): acetic acid bacteria , bacterial nanocellulose , genome sequence , Komagataeibacter diospyri and persimmon
Funding
This study was supported by the:
  • National Science and Technology Development Agency (NSTDA) (Award TG-01-52-004D)
    • Principle Award Recipient: Kallayanee Naloka
© 2020 The Authors
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2020-02-03
2024-05-14
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Komagataeibacter diospyri sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium
Int J Syst Evol Microbiol 70, 251 (2020); https://doi.org/10.1099/ijsem.0.003745
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