1887

Abstract

Two yeast strains, DMKU-WBL1-3 and DMKU GT3-16, were obtained from grease samples collected from grease traps at the Kasetsart University canteen, Thailand. Pairwise sequence analysis indicated that the strains were closely related to NRRL Y-17506, but differed by 11 and 35 nucleotide substitutions in the D1/D2 domain of the large subunit (LSU) rRNA gene and the ITS region, respectively. Based on sequence divergences, the novel species was distinguished from . The results of phylogenetic analysis based on the concatenated sequences from small subunit rRNA, ITS region and LSU rRNA genes showed that the two strains and NRRL Y-17506 formed a distinct lineage related to the genus . A novel genus, , is proposed to accommodate these clade members. Hence, NRRL Y-17506 is transferred to this genus and assigned as the type species of the genus. The holotype of is DMKU-WBL1-3.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003735
2019-10-09
2019-10-22
Loading full text...

Full text loading...

References

  1. McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W. International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code) , Regnum Vegetabile 154. Koenigstein: Koelz Scientific Books; 2012
    [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycete yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73: 331– 371 [CrossRef]
    [Google Scholar]
  3. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Berkhout In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study2011 Amsterdam: Elsevier; 1923; pp 987– 1278
    [Google Scholar]
  4. Boontham W, Limtong S, Rosa CA, Lopes MR, Vital MJS et al. Cyberlindnera tropicalis f.a., sp. nov., a novel yeast isolated from tropical regions. Int J Syst Evol Microbiol 2017;67: 2569– 2573 [CrossRef]
    [Google Scholar]
  5. Rosa CA, Lachance M-A, Teixeira LCRS, Pimenta RS, Morais PB. Metschnikowia cerradonensis sp. nov., a yeast species isolated from ephemeral flowers and their nitidulid beetles in Brazil. Int J Syst Evol Microbiol 2007;57: 161– 165 [CrossRef]
    [Google Scholar]
  6. Kurtzman C, Robnett C. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003;3: 417– 432 [CrossRef]
    [Google Scholar]
  7. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 1999;41: 95– 98
    [Google Scholar]
  8. Altschul S et al. Gapped blast and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25: 3389– 3402 [CrossRef]
    [Google Scholar]
  9. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  10. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33: 1870– 1874 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39: 783– 791 [CrossRef]
    [Google Scholar]
  12. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts In Kurtzman CP, Fell JW, Boekhout T. (editors) The yeasts, A taxonomic study2011 Amsterdam: Elsevier; 2011; pp 85– 110
    [Google Scholar]
  13. Yamada Y, Kondo K. Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 1973;19: 59– 77 [CrossRef]
    [Google Scholar]
  14. Kuraishi H, Katayama-Fujimura Y, Sugiyama J, Yokoyama T. Ubiquinone systems in fungi. I. distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implication. Trans Mycol Soc Jpn 1985;26: 385– 395
    [Google Scholar]
  15. Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 2007;57: 419– 422 [CrossRef]
    [Google Scholar]
  16. Kurtzman CP. Babjeviella Kurtzman & M. Suzuki (2010) In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study Amsterdam: Elsevier; 2010; pp 987– 1278
    [Google Scholar]
  17. Shen X-X, Opulente DA, Kominek J, Zhou X, Steenwyk JL et al. Tempo and mode of genome evolution in the budding yeast subphylum. Cell 2018;175: 1533– 1545 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003735
Loading
/content/journal/ijsem/10.1099/ijsem.0.003735
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error