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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 1

Phylogenomic analysis of and proposed reclassification to , gen. nov., comb. nov. Free

Abstract

The Gram-negative bacterium is the etiologic agent of Glässer’s disease in pigs, and causes significant economic losses to the swine industry. This bacterium has been classified as a member of the family in the genus , but phylogenetic relatedness has not been adequately examined to support this genus classification. Phenotypically, all 38 strains of tested were positive for catalase activity, oxidase activity, V-factor requirement, and acid formation from maltose and -galactose without gas. All strains were negative for X-factor requirement, formation of indole from tryptophan, urease, -arabinose, and α-glucosidase activity. To determine whether belongs to one of the current genera 40 . genomes, plus those of representative , were subjected to phylogenetic analysis of concatenated, multi-protein alignments. Sequence variation at 16S rRNA and loci allowed the 15 reference serovars of to be integrated into the whole-genome tree. The phylogenetic analysis showed to be a distinct and tight clade whose sister taxon is the genus . Within two clades were identified with individual serovars distributed between the two. As a result, was confirmed as a member of the family , but was distinct from other genera in this family. Therefore, we propose the name , gen. nov., comb. nov. for bacterial strains currently classified as . The reference strain of this species is ATCC 19417 (1374), NCTC 4557, DSM 21448, CCUG 3712.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Glaesserella parasuis , Haemophilus parasuis , phylogenomic analysis , reclassification and taxonomy
Funding
This study was supported by the:
  • National Pork Producers Council (Award NPB #12-041)
    • Principle Award Recipient: Thomas J Inzana
© 2020 The Authors
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2019-10-08
2025-04-23
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Phylogenomic analysis of Haemophilus parasuis and proposed reclassification to Glaesserella parasuis, gen. nov., comb. nov.
Int J Syst Evol Microbiol 70, 180 (2020); https://doi.org/10.1099/ijsem.0.003730
/content/journal/ijsem/10.1099/ijsem.0.003730
/content/journal/ijsem/10.1099/ijsem.0.003730
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