1887

Abstract

The genus was erected to accommodate -like species with verruculose conidia. So far, it includes only four species, which have been found in association with plants, and very little is known about their distribution and host preferences. In this study, a -like fungus isolated from sea water from the north of Portugal was characterised. Phylogenetic analysis, based on sequence data of the internal transcribed spacer and beta-tubulin loci, placed this fungus within the genus but clearly distinct from the other known species. A novel species sp. nov. is described and illustrated. The taxonomic affiliation of the genus at the family level was addressed through individual and combined gene genealogies. Our results show that the genus is a member of the family .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003680
2019-08-29
2019-10-20
Loading full text...

Full text loading...

References

  1. de Gruyter J, Aveskamp MM, Woudenberg JH, Verkley GJ, Groenewald JZ et al. Molecular phylogeny of Phoma and allied anamorph genera: towards a reclassification of the Phoma complex. Mycol Res 2009;113:508–519 [CrossRef][PubMed]
    [Google Scholar]
  2. Chen Q, Hou LW, Duan WJ, Crous PW, Cai L. Didymellaceae revisited. Stud Mycol 2017;87:105–159 [CrossRef][PubMed]
    [Google Scholar]
  3. Aveskamp MM, de Gruyter J, Woudenberg JH, Verkley GJ, Crous PW. Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera. Stud Mycol 2010;65:1–60 [CrossRef][PubMed]
    [Google Scholar]
  4. Chen Q, Jiang JR, Zhang GZ, Cai L, Crous PW. Resolving the Phoma enigma. Stud Mycol 2015;82:137–217 [CrossRef][PubMed]
    [Google Scholar]
  5. Crous PW, Groenewald JZ. They seldom occur alone. Fungal Biol 2016;120:1392–1415 [CrossRef][PubMed]
    [Google Scholar]
  6. Thambugala KM, Daranagama DA, Phillips AJL, Bulgakov TS, Bhat DJ et al. Microfungi on Tamarix. Fungal Divers 2017;82:239–306 [CrossRef]
    [Google Scholar]
  7. Wijayawardene NN, Hyde KD, Wanasinghe DN, Papizadeh M, Goonasekara ID et al. Taxonomy and phylogeny of dematiaceous coelomycetes. Fungal Divers 2016;77:1–316 [CrossRef]
    [Google Scholar]
  8. Ariyawansa HA, Hyde KD, Jayasiri SC, Buyck B, Chethana KWT et al. Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 2015;75:27–274 [CrossRef]
    [Google Scholar]
  9. Crous PW, Schumacher RK, Wingfield MJ, Lombard L, Giraldo A et al. Fungal systematics and evolution: FUSE 1. Sydowia 2015;67:81–118
    [Google Scholar]
  10. Crous PW, Wingfield MJ, Burgess TI, Carnegie AJ, Hardy G et al. Fungal planet description sheets: 625-715. Persoonia 2017;39:270–467 [CrossRef][PubMed]
    [Google Scholar]
  11. Crous PW, Wingfield MJ, Burgess TI, Hardy G, Barber PA et al. Fungal planet description sheets: 558-624. Persoonia 2017;38:240–384 [CrossRef][PubMed]
    [Google Scholar]
  12. Möller EM, Bahnweg G, Sandermann H, Geiger HH. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 1992;20:6115–6116 [CrossRef][PubMed]
    [Google Scholar]
  13. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal genes for phylogenies. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications California: Academic Press; 1990; pp.315–322
    [Google Scholar]
  14. Alves A, Phillips AJ, Henriques I, Correia A. Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting. Res Microbiol 2007;158:112–121 [CrossRef][PubMed]
    [Google Scholar]
  15. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from Filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323–1330[PubMed]
    [Google Scholar]
  16. O'Donnell K, Cigelnik E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 1997;7:103–116 [CrossRef][PubMed]
    [Google Scholar]
  17. Lopes A, Phillips AJ, Alves A. Mating type genes in the genus Neofusicoccum: mating strategies and usefulness in species delimitation. Fungal Biol 2017;121:394–404 [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  19. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  21. Rayner RW. A Mycological Colour Chart Kew: Commonwealth Mycological Institute; 1970
    [Google Scholar]
  22. Swart L, Crous PW, Denman S, Palm ME. Fungi occurring on Proteaceae. I. S Afr J Bot 1998;64:137–145 [CrossRef]
    [Google Scholar]
  23. Crous PW, Summerell BA, Swart L, Denman S, Taylor JE et al. Fungal pathogens of Proteaceae. Persoonia 2011;27:20–45 [CrossRef][PubMed]
    [Google Scholar]
  24. Picard KT. Coastal marine habitats harbor novel early-diverging fungal diversity. Fungal Ecol 2017;25:1–13 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003680
Loading
/content/journal/ijsem/10.1099/ijsem.0.003680
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error