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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 12

gen. nov., sp. nov. and sp. nov., new members of the family isolated from coastal seawater Free

Abstract

Two -like bacteria, designated strains SYSU SYW-5 and SYSU SYW-6, were isolated from coastal seawater sampled at Huizhou Double-Moon Bay, Guangdong Province, PR China. Cells were found to be Gram-stain-negative, non-motile, non-spore-forming and of pleomorphic shape (coccobacilli or rod). Chemotaxonomic analysis of the plasma membrane revealed ubiquinone-8 as the respiratory quinone, and saturated branched-chain (anteiso-C) and saturated straight-chain (C) fatty acids as major components (>8 % of total fatty acid). The major polar lipids comprised diphosphatidylglycerol (cardiolipin), phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine, as well as an unidentified aminophospholipid and an unidentified phospholipid in SYSU SYW-5, and two unknown polar lipids in SYSU SYW-6. Comparison of 16S rRNA gene sequences showed that SYSU SYW-5 had maximum similarity to SYSU HZH-2 (93.4 %), while SYSU SYW-6had maximum similarity to SYSU D3-2 (97.5 %). Phylogenetic dendrograms based on 16S rRNA genes and 31 concatenated protein markers revealed that the two novel strains represent a distinct lineage within the family . The average nucleotide identity and DNA–DNA hybridization values between the two isolates and their related species were well below the threshold limit for species delineation (<89.2 and <35 %, respectively). Based on the above results, strain SYSU SYW-5 is proposed to be a novel species of a new genus with the name gen. nov., sp. nov. (SYSU SYW-5 = DSM 103991= KCTC 52556), and strain SYSU SYW-6 is proposed to be a novel species of genus with the name sp. nov. (SYSU SYW-6=DSM 103992=KCTC 52558).

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  • Accepted:
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Keyword(s): coastal seawater , Cysteiniphilum halobium sp. nov. , Facilibium subflavum gen. nov., sp. nov. and polyphasic taxonomy
© 2019 The Authors
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2019-12-01
2024-04-27
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Facilibium subflavum gen. nov., sp. nov. and Cysteiniphilum halobium sp. nov., new members of the family Fastidiosibacteraceae isolated from coastal seawater
Int J Syst Evol Microbiol 69, 3757 (2019); https://doi.org/10.1099/ijsem.0.003676
/content/journal/ijsem/10.1099/ijsem.0.003676
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