1887

Abstract

Two -like bacteria, designated strains SYSU SYW-5 and SYSU SYW-6, were isolated from coastal seawater sampled at Huizhou Double-Moon Bay, Guangdong Province, PR China. Cells were found to be Gram-stain-negative, non-motile, non-spore-forming and of pleomorphic shape (coccobacilli or rod). Chemotaxonomic analysis of the plasma membrane revealed ubiquinone-8 as the respiratory quinone, and saturated branched-chain (anteiso-C) and saturated straight-chain (C) fatty acids as major components (>8 % of total fatty acid). The major polar lipids comprised diphosphatidylglycerol (cardiolipin), phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine, as well as an unidentified aminophospholipid and an unidentified phospholipid in SYSU SYW-5, and two unknown polar lipids in SYSU SYW-6. Comparison of 16S rRNA gene sequences showed that SYSU SYW-5 had maximum similarity to SYSU HZH-2 (93.4 %), while SYSU SYW-6had maximum similarity to SYSU D3-2 (97.5 %). Phylogenetic dendrograms based on 16S rRNA genes and 31 concatenated protein markers revealed that the two novel strains represent a distinct lineage within the family . The average nucleotide identity and DNA–DNA hybridization values between the two isolates and their related species were well below the threshold limit for species delineation (<89.2 and <35 %, respectively). Based on the above results, strain SYSU SYW-5 is proposed to be a novel species of a new genus with the name gen. nov., sp. nov. (SYSU SYW-5 = DSM 103991= KCTC 52556), and strain SYSU SYW-6 is proposed to be a novel species of genus with the name sp. nov. (SYSU SYW-6=DSM 103992=KCTC 52558).

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2019-12-01
2019-12-13
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