1887

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, rod-shaped and motile with single polar flagellum bacterial strain, designated DHC06, was isolated from forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, PR China. The strain grew at 4–37 °C (optimum, 28 °C), pH 4.5–8.0 (pH 6.0–7.5) and in the presence of 0–4.0 % (2.0 %, w/v) NaCl. In the 16S rRNA gene sequence phylogram, strain DHC06 formed a clade with DHG54 and KACC 12748 within the genus of . Strain DHC06 had 16S rRNA gene sequence similarities of 98.6, 98.3, 98.3 and 98.2 % to DSM 16301, JS14-6, KACC 12747 and DHG54, respectively. The distinctiveness of strain DHC06from all described species was also supported by the results of phylogenomic analysis based on 92 single-copy gene sequences. The DDH values among strain DHC06 and closely related species were all lower than 70 %. Strain DHC06 contained Q-8 as the only respiratory quinone. Its main fatty acids were iso-C, iso-C 9 and summed feature 3 (C 6/C 7). The DNA G+C content of strain DHC06 was 64.6 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of phenotypic, 16S rRNA gene sequence and genomic analyses and chemotaxonomic data, strain DHC06 represents a novel species of the genus , for which the name sp. nov. (type strain DHC06=GDMCC 1.1186=LMG 30090) is proposed.

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2019-08-27
2019-10-19
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