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Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterium, designated DMKU BBB3-04, was isolated from bark of mango tree . Colonies were circular, convex with entire margins when grown on nutrient agar medium for 2 days. The bacterium was motile by means of lophotrichous flagella and produced black-brown pigment. The strain grew at 12–40 °C (optimum, 28–30 °C) and at pH 6.0–8.0 (pH 6.5). Growth was observed in the presence of up to 5 % (w/v) NaCl. Strain DMKU BBB3-04 showed the highest 16S rRNA gene sequence similarity to KF707 (97.1 %), NBRC 103045 (97.0 %), MA-69 (96.9 %) and 6H33b (96.8 %), suggesting that strain DMKU BBB3-04 should be classified within the genus . Analysis of strain DMKU BBB3-04 was also performed using three housekeeping genes (, and ) and further confirmed the phylogenetic assignment of the strain. The major fatty acids found in strain DMKU BBB3-04 were summed feature 8 (C ω7/C ω6), C and summed feature 3 (C ω7/C ω6) (35, 22 and 19 %, respectively). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The respiratory quinone was Q-9. The DNA G+C content of the strain DMKU BBB3-04 was 67.6 mol%. On the basis of phenotypic characteristics, chemotaxonomic data and phylogenetic analysis, strain DMKU BBB3-04 is considered to represent a novel species in the genus . We propose the name sp. nov. for this novel species. The type strain of the novel species is DMKU BBB3-04 (=TBRC 7080=JCM 32061).

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/content/journal/ijsem/10.1099/ijsem.0.003657
2019-11-01
2020-01-29
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