1887

Abstract

A novel actinobacterium, designated strain NEAU-THZ27, was isolated from soil collected from the Cornel peak in Jiaozuo, Henan Province, PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus. The results of 16S rRNA gene sequence analysis showed that strain NEAU-THZ27 belongs to the genus and was most closely related to YPL1 (98.96 %), Q41 (98.89 %), HKI 0478 (98.86%) and S1.4 (98.85 %), similarities to other type strains of species of the genus were found to be less than 98.7 %. Phylogenetic analyses using the 16S rRNA gene sequence and multilocus sequence analysis using the concatenated gene sequences of the , , , and genes all showed that the strain formed a separate branch in the genus . The cell wall contained -diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H). Major fatty acids were iso-C, iso-C and anteiso-C, these chemotaxonomic data supported the affiliation of strain NEAU-THZ27 to the genus . The DNA G+C content was 68.0 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA–DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-THZ27 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-THZ27 (=CGMCC 4.7504=DSM 105535).

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2019-11-01
2021-10-25
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