1887

Abstract

A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, designated as strain BML3, was isolated from a sputum sample of a hospital patient in Japan. Strain BML3 grew at temperatures from 4 to 40 °C, in 1.0–7.0 % (w/v) NaCl and at pH 6.0–9.0. Results of phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA gene and , and , showed that strain BML3 was part of the group and located close to , and . Whole-genome comparisons, using average nucleotide identity and digital DNA–DNA hybridization, confirmed strain BML3 to be a distinct species among the group. Phenotypic characterization tests demonstrated that the utilization of phenylmercuric acetate could distinguish this strain from other closed species of the group. Based on genetic and phenotypic evidence, strain BML3 should be classified as a novel species, for which the name sp. nov. is proposed. The type strain is BML3 (=DSM 109244,=JCM 33395), with a DNA G+C content of 62.66 mol %.

Keyword(s): Pseudomonas and human pathogen
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/content/journal/ijsem/10.1099/ijsem.0.003623
2019-08-01
2019-09-22
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