1887

Abstract

Two novel strains (HT111-2 and HT170-2) of the genus were isolated from faecal samples collected on the Qinghai-Tibet Plateau, PR China. The isolates were Gram-stain-positive, rod-shaped, non-spore-forming bacteria with irregular circular colonies. Phylogenetic analysis and comparison of the 16S rRNA gene sequences demonstrated that the two strains form a subcluster and are closest to JCM 6256 (97.3 %) and DSM 11664 (97.2 %). Phylogenetic analysis of two housekeeping genes ( and ) found that strains HT111-2 and HT170-2 had the same closest relatives as the 16S rRNA gene sequence analysis did. The G+C content of strains HT111-2 and HT170-2 were 38.8 mol%. The values of DNA–DNA hybridization with known species were lower than the threshold (70%). Average nucleotide identity values of strain HT111-2 with JCM 6256 and DSM 11664 were 77.84 % and 76.85 %, respectively. The major fatty acids of strains HT111-2 and HT170-2 were C, Cω and C. Results of phenotypic, chemotaxonomic and phylogenetic analyses suggest strains HT111-2 and HT170-2 represent a novel species of the genus , for which the name sp. nov. is proposed with HT111-2 (=CGMCC 1.13855=KCTC 15803) as the type strain.

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2019-09-27
2019-10-15
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