1887

Abstract

A novel endophytic actinomycete strain AZ1-13 was isolated from roots of , and its taxonomic position was investigated using a polyphasic approach. Pairwise 16S rRNA gene sequence similarities of strain AZ1-13 and its closest species, PLAI1-1 and 202201, were 99.7 and 99.2 %, respectively. Phylogenetic analyses of the family based on 16S rRNA gene sequences indicated strains AZ1-13 and PLAI1-1are located within the genus . The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content. The strain AZ1-13 exhibited ANIb values of 87.4 % with PLAI1-1 and 85.1 % with 202201. Chemotaxonomic characteristics of strain AZ1-13 were consistent within the genus : cell-wall peptidoglycan of the strain contained -diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H) and MK-9(H); major cellular fatty acids were -C, 10-methyl C, C, -C and -Cω8; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids. Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13 could be distinguished from its closest neighbours, representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AZ1-13 (=KCTC 39786=NBRC 112324=JCM 32147 = TISTR 2404). This study also proposed that is transferred to the genus as comb. nov. (type strain PLAI1-1=TBRC 7644=NBRC 113144=JCM 32592).

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/content/journal/ijsem/10.1099/ijsem.0.003574
2019-09-01
2019-12-12
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