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Volume 69, Issue 8

gen. nov., sp. nov., isolated from an explosives waste contaminated site Free

Abstract

A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19, was isolated from an explosives contaminated site in Panchkula, Haryana, India. The 16S rRNA gene sequencing analysis indicated that the strain is a member of the family with the highest sequence similarity to XN13 (96.1 %), followed by S1 (95.6 %), DSM 23997 (95.6 %), ISL-41 (95.6 %), M8 (95.5 %), LCB217 (95.5 %) and DSM 17275 (95.5 %). Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family . Strain S5-TSA-19 had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on -Lys--Asp, iso-C as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera and , thus supporting the findings of molecular phylogeny. Further, strain S5-TSA-19 was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2–4 days, whereas the closest reference strains did not possess this property. On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19 is proposed as the type strain of a novel species in a novel genus for which the name gen. nov., sp. nov. is proposed (=JCM 31737=KCTC 33871=MTCC 12608).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , biotransformation , explosives , Indiicoccus , Indiicoccus explosivorum , phylogeny and S5-TSA-19T
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2019-08-01
2024-05-06
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Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site
Int J Syst Evol Microbiol 69, 2555 (2019); https://doi.org/10.1099/ijsem.0.003541
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