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Abstract

A non-motile, Gram-stain-positive, rod-shaped bacterium, designated RP-3-7, was isolated from soil sampled at the Arctic region in Cambridge Bay, NU, Canada. Cells were strictly aerobic, non-spore-forming, catalase-positive and oxidase-positive. Colonies on Reasoner's 2A agar plates were pale yellow-coloured. Strain RP-3-7 was psychrotolerant and grew optimally at 15–20 °C. Strain RP-3-7 assimilated -glucose, -mannitol, -arabinose and -proline; tolerated only 0.5 % NaCl (w/v). Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RP-3-7 formed a lineage within the family and clustered with members of the genus . The closest member was MP203 (98.26 % sequence similarity). The major respiratory quinone was MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were anteiso-C, iso-C, anteiso-C and anteiso-C A. The diagnostic amino acid was 2,4-diaminobutyric acid and whole-cell sugars were xylose, rhamnose and mannose. The DNA G+C content of strain RP-3-7 was 66.9 mol%. The average nucleotide identity and DNA–DNA relatedness values between strain RP-3-7 and MP203 were 73.79 and 20.1 %, respectively. Based on the polyphasic, genomic and phylogenetic data, strain RP-3-7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RP-3-7 (=KEMB 9005-731=KACC 21151=NBRC 113769).

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2019-08-01
2019-09-22
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