1887

Abstract

A nitrite-tolerant denitrifying bacterium, strain GL14, was isolated from the nitrification/denitrification bioreactor in our laboratory. Strain GL14 was Gram-stain-negative, rod-shaped, non-spore-forming, facultatively anaerobic and motile by means of a single polar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences indicated that it was assigned to the genus with highest 16S rRNA gene sequence similarity (98.77 %) to DSM 18231 and NEAU-ST5-5, followed by ATCC 17588 (98.42 %), HL22-2 (98.29 %) and NEAU-ST5-21 (98.22 %). Phylogenetic analysis based on both concatenated sequences of the 16S rRNA gene and two housekeeping genes ( and ) and genome sequences further clarified the intrageneric phylogenetic position of strain GL14. The DNA G+C content of GL14 was 63.1 mol%. The results of digital DNA–DNA hybridization (highest 24.2 % of DNA–DNA relatedness) based on the Genome-to-Genome Distance Calculator and average nucleotide identity analyses (highest 80.23 %) confirmed that the strain was distinctly delineated from known species of the genus . The major fatty acids were summed feature 8 (C 7/C 6), C, summed feature 3 (C 7/C 6), Ccyclo and C. The respiratory quinone was ubiquinone Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phylogenetic, genomic, phenotypic and chemotaxonomic analyses, it was concluded that strain GL14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GL14 (=CGMCC 1.13874=NBRC 113853).

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2019-08-01
2024-12-03
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