1887

Abstract

Strain CCP-18, isolated from a freshwater pond in Taiwan, was characterized by using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CCP-18 belongs to the genus and has the highest levels of sequence similarity with respect to IMCC1713 (98.9 %) and TNR-25 (98.0 %). Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed white-coloured colonies. Optimal growth occurred at 25 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-18 were summed feature 3 (comprising Cω7 and/or Cω6) and C. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids, an unidentified phospholipid, an unidentified aminolipid and an unidentified lipid. The predominant polyamine was putrescine. The major isoprenoid quinone was Q-8. The draft genome was approximately 3.76 Mb in size with a G+C content of 68.9 mol%. The DNA–DNA hybridization values for strain CCP-18with IMCC1713 and TNR-25 were less than 40 %. Based on the phylogenetic and phenotypic data, strain CCP-18 should be classified within the genus as a representative of a novel species, named sp. nov. The type strain is CCP-18 (=BCRC 81120=LMG 30595=KCTC 62511).

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2019-08-01
2019-08-25
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