1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 8

gen. nov., sp. nov., isolated from marine sediment Free

Abstract

A novel Gram-stain-negative, rod-shaped marine bacterium, designated strain F02, was isolated from a marine saltern in Weihai, PR China. The cells of strain F02 were approximately 0.8–1.0×3.0–4.0 µm and motile by means of a polar flagellum. Strain F02 grew optimally at 33–35 °C, pH 7.5 and in the presence of 3.0 % (w/v) NaCl. Strain F02 showed oxidase- and catalase-positive activities. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain F02 belonged to the family and exhibited 16S rRNA gene sequence similarities of 96.6 and 96.4 % to the type strains of and , respectively. The major cellular fatty acids of strain F02 were Cω7, Cω7, C and Cω9. The major polar lipids of strain F02 were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Strain F02 contained Q-8 as the sole respiratory quinone. The genomic DNA G+C content was 58.4 mol%. The genome sequences of strain F02 and DSM 23344 had an OrthoANI value of 70.5 %, and the average amino acid identity value between the two genomes was 62.6 %. The sequence similarity value between the genes of strain F02 and DSM 23344 was 79.5 %. On the basis of polyphasic analysis, strain F02 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is F02 (=KCTC 52616=MCCC 1H00224).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): draft genome sequence , Halieaceae and phylogenetic analysis
© 2019 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003492
2019-08-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/8/2395.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003492&mimeType=html&fmt=ahah

References

  1. Spring S, Scheuner C, Göker M, Klenk HP. A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data. Front Microbiol 2015; 6:281 [View Article][PubMed]
     [Google Scholar]
  2. Lucena T, Pascual J, Garay E, Arahal DR, MacIán MC et al. Haliea mediterranea sp. nov., a marine gammaproteobacterium. Int J Syst Evol Microbiol 2010; 60:1844–1848 [View Article][PubMed]
     [Google Scholar]
  3. Spring S, Riedel T, Spröer C, Yan S, Harder J et al. Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans . BMC Microbiol 2013; 13:118 [View Article][PubMed]
     [Google Scholar]
  4. Shi MJ, Wang C, Wang XT, Du ZJ. Halioglobus lutimaris sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2018; 68:876–880 [View Article][PubMed]
     [Google Scholar]
  5. Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:230 [View Article][PubMed]
     [Google Scholar]
  6. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans . Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
     [Google Scholar]
  7. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  9. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  10. Csotonyi JT, Stackebrandt E, Swiderski J, Schumann P, Yurkov V. Chromocurvus halotolerans gen. nov., sp. nov., a gammaproteobacterial obligately aerobic anoxygenic phototroph, isolated from a Canadian hypersaline spring. Arch Microbiol 2011; 193:573–582 [View Article][PubMed]
     [Google Scholar]
  11. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  12. Konstantinidis KT, Rosselló-Móra R, Amann R. Uncultivated microbes in need of their own taxonomy. Isme J 2017; 11:2399–2406 [View Article][PubMed]
     [Google Scholar]
  13. Adékambi T, Shinnick TM, Raoult D, Drancourt M. Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation. Int J Syst Evol Microbiol 2008; 58:1807–1814 [View Article][PubMed]
     [Google Scholar]
  14. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  15. Dong XZ, Cai MY. Determination of Biochemical Characteristics. Manual for the Systematic Identification of General Bacteria Beijing: Science Press (In Chinese); 2001 pp. 370–398
     [Google Scholar]
  16. Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M et al. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense . Int J Syst Evol Microbiol 2007; 57:1095–1107 [View Article][PubMed]
     [Google Scholar]
  17. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [View Article][PubMed]
     [Google Scholar]
  18. CLSI Performance Standards for Antimicrobial Susceptibility Testing, 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute; 2012
     [Google Scholar]
  19. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  20. Collins MD, Goodfellow M, Minnikin DE. Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 1980; 118:29–37 [View Article][PubMed]
     [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  22. Xu XW, Wu YH, Wang CS, Oren A, Zhou PJ et al. Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 2007; 57:717–720 [View Article][PubMed]
     [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. MIDI Inc: Newark, DE; 1990
     [Google Scholar]
  24. Lin CY, Zhang XY, Liu A, Liu C, Song XY et al. Haliea atlantica sp. nov., isolated from seawater, transfer of Haliea mediterranea to Parahaliea gen. nov. as Parahaliea mediterranea comb. nov. and emended description of the genus Haliea . Int J Syst Evol Microbiol 2015; 65:3413–3418 [View Article][PubMed]
     [Google Scholar]
  25. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Haliea rubra sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 2009; 59:1188–1192 [View Article][PubMed]
     [Google Scholar]
  26. Spring S, Lünsdorf H, Fuchs BM, Tindall BJ. The photosynthetic apparatus and its regulation in the aerobic gammaproteobacterium Congregibacter litoralis gen. nov., sp. nov. PLoS One 2009; 4:e4866 [View Article][PubMed]
     [Google Scholar]
  27. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Haliea salexigens gen. nov., sp. nov., a member of the Gammaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 2008; 58:1233–1237 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.003492
Loading
Kineobactrum sediminis gen. nov., sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 69, 2395 (2019); https://doi.org/10.1099/ijsem.0.003492
/content/journal/ijsem/10.1099/ijsem.0.003492
/content/journal/ijsem/10.1099/ijsem.0.003492
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error