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Abstract

Strain WWJ-16 was isolated from a freshwater reservoir in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain WWJ-16 is affiliated with the species in the genus . Strain WWJ-16 was most closely related to MIC3010 (97.9 % 16S rRNA gene sequence identity) and CMJ-5 (97.6 %). Strain WWJ-16 showed 77.4 % average nucleotide identity and 20.6 % digital DNA–DNA hybridization identity with MIC3010. Cells of strain WWJ-16 were Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and formed yellow colonies. Optimal growth occurred at 25 °C, pH 7 and in 0.5–1 % NaCl (w/v). Strain WWJ-16 contained iso-C G and iso-C as predominant fatty acids. The major hydroxyl fatty acids were iso-C 3-OH, iso-C 3-OH and iso-C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids and four uncharacterized phospholipids. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The genomic DNA G+C content of strain WWJ-16 was 39.4 mol%, as determined by genome sequencing. The genotypic and phenotypic characteristics indicate that strain WWJ-16 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WWJ-16 (=BCRC 81125 =LMG 30600=KCTC 62515).

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2019-08-01
2019-08-25
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