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Abstract

A Gram-stain-positive, strictly aerobic, rod-shaped, motile, endospore-forming strain, SYSU K30001, was isolated from a soil sample collected from a cave in Xingyi county, Guizhou province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU K30001 belonged to the genus , with the highest sequence similarity to the type strain of (98.1 %). Growth occurred at pH 6.0–9.0 (optimum, pH 7.0), at 28–55 °C (optimum, 28 °C) and in the presence of 0–3 % (w/v) NaCl (optimum in the absence of NaCl). Strain SYSU K30001 contained -2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-7 as the only isoprenoid quinone present. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. The major fatty acids were iso-C, iso-C 10, anteiso-C and iso-C. The genome G+C content was 39.8 mol%. The average nucleotide identity values between SYSU K30001 and DSM 19096 were 72.1 % (ANIb) and 83.1 % (ANIm), which were below the cut-off level (95–96 %) for species delineation. Based on phenotypic, chemotaxonomic and molecular characterizations, strain SYSU K30001 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU K30001 (=KCTC 33954=CGMCC 1.13871).

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2019-08-01
2019-10-14
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