1887

Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped, gray-pigmented bacterial strain, designated L-25–5 w-1, was isolated from the water at Baiyang Lake, PR China. Cells of strain L-25–5 w-1 were motile, with a single polar flagellum. L-25–5 w-1 was able to grow at 15–37 °C (optimum 37 °C) and pH 5–8 (optimum pH 6) in R2A medium. 16S rRNA gene sequence analysis and phylogenetic analysis of L-25–5 w-1 showed the highest relationship to Azospirillum doebereinerae GSF71 (97.9 %), Azospirillum . thiophilum DSM 21654 (97.9 %) and Azospirillum . agricola CC-HIH038 (97.0 %), with other species of the genus Azospirillum showing less than 97 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids; the major cellular fatty acids were C16 : 0, C16 : 0 3-OH, C18 : 1 2-OH, iso-C18 : 0, summed feature 2 (C12 : 0 aldehyde and/or unknown 10.9525 and/or iso-C16 : 1I and/or C14 : 0 3-OH), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c); and the major respiratory quinone was ubiquinone 10. The draft genome size of L-25–5 w-1 was 5.8 Mbp, and the DNA G+C content was 66.6 %. The average nucleotide identity value and digital DNA–DNA hybridization relatedness value between L-25–5 w-1 and related type strains were 80.2 and 24.7 % with A. doebereinerae GSF71 and 78.8 and 23.6 % with A. thiophilum DSM 21654, respectively. According to the phylogenetic, chemotaxonomic and genotypic properties, strain L-25–5 w-1 represents a novel species in the genus Azospirillum , for which the name Azospirillum griseum sp. nov. is proposed. The type strain is L-25–5 w-1 (=CGMCC 1.13672=KCTC 62777).

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/content/journal/ijsem/10.1099/ijsem.0.003460
2019-05-28
2019-08-22
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References

  1. Tarrand JJ, Krieg NR, Döbereiner J. A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 1978;24: 967– 980 [CrossRef] [PubMed]
    [Google Scholar]
  2. Lin SY, Hameed A, Liu YC, Hsu YH, Lai WA et al. Azospirillum soli sp. nov., a nitrogen-fixing species isolated from agricultural soil. Int J Syst Evol Microbiol 2015;65: 4601– 4607 [CrossRef] [PubMed]
    [Google Scholar]
  3. Xu Y, Xu X, Lan R, Xiong Y, Ye C et al. An O island 172 encoded RNA helicase regulates the motility of Escherichia coli O157:H7. PLoS One 2013;8: e64211 [CrossRef] [PubMed]
    [Google Scholar]
  4. Gordon RE, Barnett DA, Handerhan JE, Pang CH. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974;24: 54– 63 [CrossRef]
    [Google Scholar]
  5. Liu Q, Liu HC, Zhang JL, Zhou YG, Xin YH. Rufibacter glacialis sp. nov., a psychrotolerant bacterium isolated from glacier soil. Int J Syst Evol Microbiol 2016;66: 315– 318 [CrossRef] [PubMed]
    [Google Scholar]
  6. Kamekura M. Lipids of extreme halophiles. In The Biology of Halophilic Bacteria 1993; pp. 135– 161
    [Google Scholar]
  7. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology 2007; pp. 330– 393
    [Google Scholar]
  8. Collins MD. Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics 1985; pp. 267– 287
    [Google Scholar]
  9. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 2006; 2359– 2367
    [Google Scholar]
  10. Hu YT, Zhou PJ, Zhou YG, Liu ZH, Liu SJ. Saccharothrix xinjiangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjiang, China. Int J Syst Evol Microbiol 2004;54: 2091– 2094 [CrossRef] [PubMed]
    [Google Scholar]
  11. Hou Q, Bai X, Li W, Gao X, Zhang F et al. Design of primers for evaluation of lactic acid bacteria populations in complex biological samples. Front Microbiol 2018;9: 9 [CrossRef] [PubMed]
    [Google Scholar]
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425 [CrossRef] [PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20: 406– 416 [CrossRef]
    [Google Scholar]
  16. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64: 346– 351 [CrossRef] [PubMed]
    [Google Scholar]
  17. Zerbino DR, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 2008;18: 821– 829 [CrossRef] [PubMed]
    [Google Scholar]
  18. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  19. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14: 60 [CrossRef] [PubMed]
    [Google Scholar]
  20. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007;57: 81– 91 [CrossRef] [PubMed]
    [Google Scholar]
  21. Eckert B, Weber OB, Kirchhof G, Halbritter A, Stoffels M et al. Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus. Int J Syst Evol Microbiol 2001;51: 17– 26 [CrossRef] [PubMed]
    [Google Scholar]
  22. Mehnaz S, Weselowski B, Lazarovits G. Azospirillum zeae sp. nov., a diazotrophic bacterium isolated from rhizosphere soil of Zea mays. Int J Syst Evol Microbiol 2007;57: 2805– 2809 [CrossRef] [PubMed]
    [Google Scholar]
  23. Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA et al. Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter. Int J Syst Evol Microbiol 2013;63: 3762– 3768 [CrossRef] [PubMed]
    [Google Scholar]
  24. Lin SY, Liu YC, Hameed A, Hsu YH, Huang HI et al. Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil. Int J Syst Evol Microbiol 2016;66: 1453– 1458 [CrossRef] [PubMed]
    [Google Scholar]
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