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Abstract

A novel actinomycete isolate, designated strain MK-45, was isolated from soil sampled at a manganese-contaminated area in Xiangtan, China. The isolate formed extensively branched substrate mycelia and aerial mycelia that differentiated into tightly coiled or spiral spore chains with smooth-surfaced spores. The cell-wall hydrolysates contained -diaminopimelic acid and traces of -diaminopimelic acid. The major menaquinones consisted of MK-9(H), MK-9(H) and MK-9(H). The major polar lipids contained diphosphatidylgycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphotidylinositol and phosphotidylinositol mannosides. The predominant cellular fatty acids were iso-C, C and summed feature 3. Phylogenetic analysis based on the 16S rRNA gene sequences and concatenated partial sequences of the five protein-coding genes indicated that strain MK-45 was a member of the genus and most closely related to GIMN4.002 (99.5 % similarity), CGMCC 4.1680 (99.5 %), CCNWHQ 0031 (99.4 %) and NRRL B12382 (98.9 %), respectively. However, the digital DNA–DNA hybridization values, the average nucleotide identity values and MLSA evolutionary distances between strain MK-45 and them showed that it belonged to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain MK-45 from the four closely related type strains mentioned above and other species of the genus with which this strain has 98.0–99.2 % 16S rRNA gene sequence similarity. Therefore, it is concluded that strain MK-45 represents a novel species of the genus , for which the name sp. nov. is proposed, with MK-45 (=CICC 11045=KCTC 49099) as the type strain.

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2019-08-01
2019-12-12
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