1887

Abstract

A novel actinomycete, designated as strain H219, was isolated from rhizosphere soil collected under an Elephant ear plant (Colocasiaesculenta) in Bangkok, Thailand. Strain H219 was characterised using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequences revealed that this isolate was most closely related to Saccharopolyspora tripterygii JCM 32123 (97.6 %), Saccharopolyspora dendranthemae NBRC 108675 (97.5 %) and Saccharopolyspora flava NBRC 16345 (97.5 %). However, DNA–DNA hybridization analyses showed a low relatedness in the range of 39–48 % between the novel isolate and the above closely related strains. The cell-wall peptidoglycan of strain H219 contained meso-diaminopimelic acid. The diagnostic whole-cell sugars consisted of arabinose and galactose. The cellular fatty acid profile mainly comprised iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, and 10-methyl C17 : 0. The major menaquinone was MK-9(H4). The detected phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid. The DNA G+C content was 70.6 mol%. Strain H219 represented chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora . However, strain H219 could be distinguished from closely related strains by several phenotypic properties. Based on the data from the polyphasic studies, we propose that strain H219 is a novel species within the genus Saccharopolyspora , Saccharopolyspora rhizosphaerae sp. nov. The type strain is H219 (=TBRC 8564=NBRC 113388).

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2019-02-21
2020-01-24
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