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Volume 69, Issue 4

gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order ord. nov. within the class of the phylum Free

Abstract

A novel slow-growing, facultatively anaerobic, filamentous bacterium, strain MO-CFX2, was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediment collected off the Shimokita Peninsula of Japan. Cells were multicellular filamentous, non-motile and Gram-stain-negative. The filaments were generally more than 20 µm (up to approximately 200 µm) long and 0.5–0.6 µm wide. Cells possessed pili-like structures on the cell surface and a multilayer structure in the cytoplasm. Growth of the strain was observed at 20–37 °C (optimum, 30 °C), pH 5.5–8.0 (pH 6.5–7.0), and 0–30 g l NaCl (5 g l NaCl). Under optimum growth conditions, doubling time and maximum cell density were estimated to be approximately 19 days and ~10 cells ml, respectively. Strain MO-CFX2 grew chemoorganotrophically on a limited range of organic substrates in anaerobic conditions. The major cellular fatty acids were saturated C (47.9 %) and C (36.9 %), and unsaturated Cω9c (6.0 %) and Cω7 (5.1 %). The G+C content of genomic DNA was 63.2 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-CFX2 shares a notably low sequence identity with its closest relatives, which were GNS-1 and SW7 (both 85.8 % sequence identity). Based on these phenotypic and genomic properties, we propose the name gen. nov., sp. nov. for strain MO-CFX2 (=KCTC 15625, =JCM 32065). In addition, we also propose the associated family and order as fam. nov. and ord. nov., respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aggregatilinea lenta , Anaerolineae , Chloroflexi , novel species and subseafloor sediment
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP1610845)
  • Japan Society for the Promotion of Science (Award 15H02419)
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2019-02-18
2023-03-28
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Aggregatilinea lenta gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order Aggregatilineales ord. nov. within the class Anaerolineae of the phylum Chloroflexi
Int J Syst Evol Microbiol 69, 1185 (2019); https://doi.org/10.1099/ijsem.0.003291
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