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Volume 69, Issue 4

sp. nov., isolated from deep-sea sediment of the Indian Ocean and proposal of fam. nov Free

Abstract

A Gram-staining negative, aerobic, oval-shaped bacterium, designated strain PTG4-2, was isolated from deep-sea sediment of the Indian Ocean. Growth was observed with 1–9 % (w/v) NaCl with optimal growth with 3 %, at pH 6.0–10.0 with an optimum of pH 7.0, and at 4–40 °C with an optimum of 30 °C. Positive for catalase and oxidase. The results of a 16S rRNA gene sequence comparison indicated that PTG4-2 was most closely related to JL1095 (97.3 %), followed by J103 (96.5 %), all other species shared <93 % sequence similarity. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that PTG4-2 forms a distinct lineage within the genus , and revealed that the genus forms a novel family-level clade in the order . The ANI and the DNA–DNA hybridization estimate values between PTG4-2 and two type strains ( JL1095 and J103) were 79.9–76.2 % and 23.1–20.8 %, respectively. PTG4-2 contained Q-10 as the predominant ubiquinone. The principal fatty acids (>5 %) were summed feature 8 [C 7/6 (72.2 %)], C (8.4 %), C 7 (6.4 %) and C (6.3 %). The polar lipids consisted of phosphatidylglycerol, three unidentified phospholipids, two unidentified glycolipids, one unidentified aminolipid and one unknown lipid. The DNA G+C content of PTG4-2 is 69.2 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, PTG4-2 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed, with the type strain PTG4-2 (=MCCC 1A01274=KCTC 52323). In addition, a novel family, fam. nov., is proposed to accommodate the genus .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acuticoccaceae fam. nov. , Acuticoccus sediminis sp. nov. , deep-sea sediment and Indian Ocean
Funding
This study was supported by the:
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award 16PZP001SF16)
  • China Ocean Mineral Resources RandD Association program (Award No. DY135-B2-01)
  • National Infrastructure of Microbial Resources of China (Award NIMR-2018-9)
© 2019 IUMS
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2019-02-27
2024-05-13
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Acuticoccus sediminis sp. nov., isolated from deep-sea sediment of the Indian Ocean and proposal of Acuticoccaceae fam. nov
Int J Syst Evol Microbiol 69, 1173 (2019); https://doi.org/10.1099/ijsem.0.003289
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