1887

Abstract

Cells of bacterial strains 4 G-K06 and 4MSK11, isolated from soil samples collected from monsoon evergreen broad-leaved forest of the Dinghushan Mountain (112° 31′ E 23° 10′ N), Guangdong Province, PR China, were Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped. Strain 4 G-K06 grew at 10–37 °C, pH 3.5–7.5 and 0–3.5 % (w/v) NaCl; while 4MSK11 grew at 4–42 °C, pH 3.5–7.5 and 0–2.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed strain 4 G-K06 formed a clade with 4 M-K16, 4M-Z03, DHG40 and DHG59, while strain 4MSK11 formed a clade with DHOB09 and DHON07, both within the genus . The result of the partial , and gene sequence analysis supported the conclusion based on 16S rRNA gene sequence analysis, which showed that these two strains represent two novel species of . The average nucleotide identity and digital DNA–DNA hybridization value for the whole genomes were 75.0–79.0 and 20.3–22.6 % between strains 4 G-K06, 4MSK11 and those described species with genome sequences; while the DNA–DNA hybridization rates between strains 4 G-K06, 4MSK11 and closely related species (without genome sequence) were 29.5–41.8 %. The major cellular fatty acids of these two strains were iso-C, iso-C and iso-C 9, while the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids and aminophospholipids. The only ubiquinone of these two strains was ubiquinone-8. The DNA G+C contents of 4 G-K06 and 4MSK11 were 60.4 and 61.3 mol%, respectively. On the basis of the evidence presented here, strains 4 G-K06 and 4MSK11 represent two novel species of the genus , for which the names sp. nov. (type strain 4 G-K06=LMG 30268=GDMCC 1.1188) and sp. nov. (type strain 4MSK11=KCTC 62280=GDMCC 1.1185) are proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award J1310025)
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2019-01-31
2021-05-13
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