1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 3

Motilimonas pumila sp. nov., isolated from the gut of sea cucumber Apostichopus japonicus Free

Abstract

A facultatively anaerobic and Gram-negative bacterium, designated strain PLHSC7-2, was isolated from the gut of sea cucumber Apostichopusjaponicus that had been collected from the coastal area of Yantai, China. The cells were rod-shaped and motile by means of polar flagella. The novel isolate grew optimally at 28–30 °C, in the presence of 2.0–3.0 % (w/v) NaCl and at pH 7.0–7.5. The sole respiratory quinone was Q-8 and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C17 : 0. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLHSC7-2 was phylogenetically affiliated with the genus Motilimonas , and exhibited sequence similarity of 96.2 % to Motilimonas eburnea YH6. The DNA G+C content was 45.5 mol%. On the basis of phenotypic , phylogenetic and genetic distinctiveness, strain PLHSC7-2 (=MCCC 1K03522=KCTC 62589) was classified as a novel species within the genus Motilimonas , for which the name Motilimonas pumila sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , gut of sea cucumber , Motilimonas pumila sp. nov. and polyphasic study
© 2019 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003242
2019-01-28
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/3/811.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003242&mimeType=html&fmt=ahah

References

  1. Zhang X, Nakahara T, Murase S, Nakata H, Inoue T et al. Physiological characterization of aerobic culturable bacteria in the intestine of the sea cucumber Apostichopus japonicus. J Gen Appl Microbiol 2013; 59:1–10 [View Article][PubMed]
     [Google Scholar]
  2. Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018; 6:230 [View Article][PubMed]
     [Google Scholar]
  3. Du ZJ, Miao TT, Rooney AP, Liu QQ, Chen GJ. Neiella marina gen. nov., sp. nov., isolated from the sea cucumber Apostichopus japonicus. Int J Syst Evol Microbiol 2013; 63:1597–1601 [View Article][PubMed]
     [Google Scholar]
  4. Song EJ, Lee MH, Seo MJ, Yim KJ, Hyun DW et al. Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 2014; 106:837–844 [View Article][PubMed]
     [Google Scholar]
  5. Wang L, Li X, Hu D, Lai Q, Shao Z. Tenacibaculum holothuriorum sp. nov., isolated from the sea cucumber Apostichopus japonicus intestine. Int J Syst Evol Microbiol 2015; 65:4347–4352 [View Article][PubMed]
     [Google Scholar]
  6. Lee MH, Song EJ, Seo MJ, Hyun DW, Bae JW et al. Phaeobacter marinintestinus sp. nov., isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 2015; 107:209–216 [View Article][PubMed]
     [Google Scholar]
  7. Amaro T, Witte H, Herndl GJ, Cunha MR, Billett DSM. Deep-sea bacterial communities in sediments and guts of deposit-feeding holothurians in Portuguese canyons (NE Atlantic). Deep Sea Res 2009; 56:1834–1843 [View Article]
     [Google Scholar]
  8. Enomoto M, Nakagawa S, Sawabe T. Microbial communities associated with holothurians: presence of unique bacteria in the coelomic fluid. Microbes Environ 2012; 27:300–305 [View Article][PubMed]
     [Google Scholar]
  9. Bowman JP, McMeekin TA, Order X. Alteromonadales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. New York: Springer; 2005 pp. 443
     [Google Scholar]
  10. Ling SK, Guo LY, Chen GJ, Du ZJ. Motilimonas eburnea gen. nov., sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2017; 67:306–310 [View Article][PubMed]
     [Google Scholar]
  11. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
     [Google Scholar]
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  15. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article][PubMed]
     [Google Scholar]
  16. Jackman SD, Vandervalk BP, Mohamadi H, Chu J, Yeo S et al. ABySS 2.0: resource-efficient assembly of large genomes using a Bloom filter. Genome Res 2017; 27:768–777 [View Article][PubMed]
     [Google Scholar]
  17. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
  18. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  19. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  20. Dong XZ, Cai MY. (editors) Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp. 370–398
     [Google Scholar]
  21. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [View Article][PubMed]
     [Google Scholar]
  22. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI, Inc; 1990
     [Google Scholar]
  23. Collins MD. Isoprenoid quinone analysis in classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–287
     [Google Scholar]
  24. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
     [Google Scholar]
  25. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003242
Loading
Motilimonas pumila sp. nov., isolated from the gut of sea cucumber Apostichopus japonicus
Int J Syst Evol Microbiol 69, 811 (2019); https://doi.org/10.1099/ijsem.0.003242
/content/journal/ijsem/10.1099/ijsem.0.003242
/content/journal/ijsem/10.1099/ijsem.0.003242
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error