1887

Abstract

An arsenic-resistant strain, CB3, was isolated from arsenic-rich aquifers at the Jianghan Plain in Hubei, China. Phylogenetic and biochemical analysis suggested that it should represent a new species of the genus Pseudaminobacter in the family Phyllobacteriaceae. The 16S rRNA gene of CB3 shared the highest sequence similarities to those of the type strains Pseudaminobacter defluvii THI 051 (97.8 % identity) and Pseudaminobacter salicylatoxidans BN12 (97.4 %). The DNA–DNA relatedness values of CB3 with respect to strains belonging to the genus Pseudaminobacter were less than 70 %. The fatty acid profile of CB3 consisted of C16 : 0, cyclo-C19 : 0 ω8c and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) as major components. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 61.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CB3 was distinct from previously described Pseudaminobacter species. Therefore, we propose that strain CB3 represents a novel species of the genus Pseudaminobacter, Pseudaminobacter arsenicus sp. nov., strain CB3 (=CCTCC AB2016116=KCTC 52625) is designated as the type strain.

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2019-01-24
2020-01-20
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