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Volume 69, Issue 4

gen. nov., sp. nov., a new member of the clade isolated from Loki’s Castle Vent Field on the Arctic Mid-Ocean Ridge. Free

Abstract

A bacterial strain, designated BAR1, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1 were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5–8.0 (optimum pH 6.5–7.5) and 0.5–5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was Q-10, the fatty acid profile was dominated by Cω7 (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1 clustered together with PRT1, as well as the genera and in a polyphyletic clade within the clade. Several characteristics differentiate strain BAR1 from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 °C and under anaerobic conditions. Accordingly, strain BAR1 is considered to represent a novel genus and species within the clade, for which the name gen. nov., sp. nov. is proposed. The type strain is BAR1 (=JCM 31874=DSM 104147).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): alphaproteobacteria , deep-sea , hydrothermal vent , isolate , piezophilic and roseobacter
Funding
This study was supported by the:
  • Norwegian Research Council (Award 179560)
© 2019 IUMS
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2019-02-13
2024-04-26
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References

  1. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae . In Rosenberg E, Delong EF, Lory S, Stackebrandt E, Thompson FL. (editors) The Prokaryotes-Alphaproteobacteria and Betaproteobacteria Springer Verlag; 2014 pp. 439–512
     [Google Scholar]
  2. Luo H, Moran MA. Evolutionary ecology of the marine Roseobacter clade. Microbiol Mol Biol Rev 2014; 78:573–587 [View Article][PubMed]
     [Google Scholar]
  3. Brinkhoff T, Giebel HA, Simon M. Diversity, ecology, and genomics of the Roseobacter clade: a short overview. Arch Microbiol 2008; 189:531–539 [View Article][PubMed]
     [Google Scholar]
  4. Kim YO, Park S, Kim H, Park DS, Nam BH et al. Halocynthiibacter namhaensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from sea squirt Halocynthia roretzi . Antonie van Leeuwenhoek 2014; 105:881–889 [View Article][PubMed]
     [Google Scholar]
  5. Baek K, Lee YM, Shin SC, Hwang K, Hwang CY et al. Halocynthiibacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 2015; 65:3861–3865 [View Article][PubMed]
     [Google Scholar]
  6. Won SM, Park S, Park JM, Kim BC, Yoon JH. Pseudohalocynthiibacter aestuariivivens gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2015; 65:1509–1514 [View Article][PubMed]
     [Google Scholar]
  7. Pedersen RB, Rapp HT, Thorseth IH, Lilley MD, Barriga FJ et al. Discovery of a black smoker vent field and vent fauna at the Arctic Mid-Ocean Ridge. Nat Commun 2010; 1:126 [View Article][PubMed]
     [Google Scholar]
  8. Steen IH, Dahle H, Stokke R, Roalkvam I, Daae F-L et al. Novel Barite Chimneys at the Loki’s Castle Vent Field Shed light on key factors shaping microbial communities and functions in hydrothermal systems. Extreme Microbiol 2016; 6:1510
     [Google Scholar]
  9. Emerson D, Floyd MM. Enrichment and isolation of iron-oxidizing bacteria at neutral pH. Methods Enzymol 2005; 397:112–123 [View Article][PubMed]
     [Google Scholar]
  10. Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res 2016; 44:D67–D72 [View Article][PubMed]
     [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  12. Eloe EA, Malfatti F, Gutierrez J, Hardy K, Schmidt WE et al. Isolation and characterization of a psychropiezophilic alphaproteobacterium. Appl Environ Microbiol 2011; 77:8145–8153 [View Article][PubMed]
     [Google Scholar]
  13. Fisher RA. On the “Probable Error” of a coefficient of correlation deduced from a small sample. Metron 1921; 1:205–235
     [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  15. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
     [Google Scholar]
  16. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–IN1 [View Article]
     [Google Scholar]
  17. Roalkvam I, Bredy F, Baumberger T, Pedersen RB, Steen IH. Hypnocyclicus thermotrophus gen. nov., sp. nov. isolated from a microbial mat in a hydrothermal vent field. Int J Syst Evol Microbiol 2015; 65:4521–4525 [View Article][PubMed]
     [Google Scholar]
  18. Haft DH, Dicuccio M, Badretdin A, Brover V, Chetvernin V et al. RefSeq: an update on prokaryotic genome annotation and curation. Nucleic Acids Res 2018; 46:D851–D860 [View Article][PubMed]
     [Google Scholar]
  19. Ryu E. On the gram-differentiation of bacteria by the simplest method. J Vet Med Sci 1938; 17:205–207 [View Article]
     [Google Scholar]
  20. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. Methods Gen Mol Microbiol, Third ed. 2007
     [Google Scholar]
  21. Heimbrook ME, Wang WL, Campbell G. Staining bacterial flagella easily. J Clin Microbiol 1989; 27:2612–2615[PubMed]
     [Google Scholar]
  22. Ryu E. A simple method of staining bacterial flagella. Kitasato Arch Exp Med 1937; 14:218–219
     [Google Scholar]
  23. Høvik Hansen G, Sørheim R. Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 1991; 13:231–241 [View Article]
     [Google Scholar]
  24. Jorgensen JH, Turnidge JD. Susceptibility test methods: dilution and disk diffusion methods. Man Clin Microbiol Elev Ed 2015
     [Google Scholar]
  25. Geng H, Bruhn JB, Nielsen KF, Gram L, Belas R. Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters. Appl Environ Microbiol 2008; 74:1535–1545 [View Article][PubMed]
     [Google Scholar]
  26. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In Starr MP, Stolp H, Trüper G, Balows A, Schlegel HG. (editors) The Prokaryotes Berlin: Springer; 1981 pp. 1302–1331
     [Google Scholar]
  27. Le Moine Bauer S, Roalkvam I, Steen IH, Dahle H. Lutibacter profundi sp. nov., isolated from a deep-sea hydrothermal system on the arctic mid-ocean ridge and emended description of the genus Lutibacter. Int J Syst Evol Microbiol 2016; 66:2671–2677 [View Article][PubMed]
     [Google Scholar]
  28. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  29. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
     [Google Scholar]
  30. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586[PubMed]
     [Google Scholar]
  31. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 1988; 38:358–361 [View Article]
     [Google Scholar]
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Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki’s Castle Vent Field on the Arctic Mid-Ocean Ridge.
Int J Syst Evol Microbiol 69, 975 (2019); https://doi.org/10.1099/ijsem.0.003234
/content/journal/ijsem/10.1099/ijsem.0.003234
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