1887

Abstract

A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7) was isolated from seawater. Growth occurred at 10–30 °C (optimum 25 °C), at pH 6–8 (optimum 7) and in the presence of 1–8 % (w/v) NaCl (optimum 4 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-3.7 were identified as Paraglaciecola mesophila DSM 15026 (95.3 % similarity), Glaciecola pallidula DSM 14239 (95.2 %), Paraglaciecola aquimarina KCTC 32108 (95.1 %), Paraglaciecola arctica KACC 14537 (94.9 %), Glaciecola nitratireducens KCTC 12276 (94.7 %) and Paraglaciecola psychrophila CGMCC 1.6130 (94.7 %). 16S rRNA gene sequence similarities among strain THG-3.7 and other species were lower than 94.7 %. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The quinone system was composed of Q-8. The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain THG-3.7 was 47.9 mol%. On the basis of the data presented, strain THG-3.7 represents a novel species of the genus Glaciecola , for which the name Glaciecola amylolytica sp. nov. is proposed. The type strain is THG-3.7 (=KACC 19478=CCTCC AB 2017258).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003222
2019-01-04
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/4/957.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003222&mimeType=html&fmt=ahah

References

  1. Bowman JP, McCammon SA, Brown JL, McMeekin TA. Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 1998;48:1213–1222 [CrossRef]
    [Google Scholar]
  2. Romanenko LA, Zhukova NV, Rohde M, Lysenko AM, Mikhailov VV et al. Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 2003;53:647–651 [CrossRef][PubMed]
    [Google Scholar]
  3. Yong JJ, Park SJ, Kim HJ, Rhee SK. Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 2007;57:951–953 [CrossRef][PubMed]
    [Google Scholar]
  4. Park S, Yoon JH. Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 2013;103:1141–1148 [CrossRef][PubMed]
    [Google Scholar]
  5. Zhang YJ, Zhang XY, Mi ZH, Chen CX, Gao ZM et al. Glaciecola arctica sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 2011;61:2338–2341 [CrossRef][PubMed]
    [Google Scholar]
  6. Matsuyama H, Hirabayashi T, Kasahara H, Minami H, Hoshino T et al. Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 2006;56:2883–2886 [CrossRef][PubMed]
    [Google Scholar]
  7. van Trappen S, Tan TL, Yang J, Mergaert J, Swings J. Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola. Int J Syst Evol Microbiol 2004;54:1765–1771 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhang DC, Yu Y, Chen B, Wang HX, Liu HC et al. Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 2006;56:2867–2869 [CrossRef][PubMed]
    [Google Scholar]
  9. Shivaji S, Reddy GS. Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov. as Paraglaciecola mesophila comb. nov., P. agarilytica comb. nov., P. aquimarina comb. nov., P. arctica comb. nov., P. chathamensis comb. nov., P. polaris comb. nov. and P. psychrophila comb. nov., and description of Paraglaciecola oceanifecundans sp. nov., isolated from the Southern Ocean. Int J Syst Evol Microbiol 2014;64:3264–3275 [CrossRef][PubMed]
    [Google Scholar]
  10. Baik KS, Park YD, Seong CN, Kim EM, Bae KS et al. Glaciecola nitratireducens sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2006;56:2185–2188 [CrossRef][PubMed]
    [Google Scholar]
  11. Chen LP, Xu HY, Fu SZ, Fan HX, Liu YH et al. Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. Int J Syst Evol Microbiol 2009;59:73–76 [CrossRef][PubMed]
    [Google Scholar]
  12. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  13. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  16. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Biol 1969;18:1–32 [CrossRef]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  18. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982;44:992–993[PubMed]
    [Google Scholar]
  19. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178:703 [CrossRef][PubMed]
    [Google Scholar]
  20. Yan ZF, Lin P, Chu X, Kook M, Li CT, Ct L et al. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Arch Microbiol 2016;198:423–427 [CrossRef][PubMed]
    [Google Scholar]
  21. Yan ZF, Trinh H, Moya G, Lin P, Li CT, Ct L et al. Lysobacter rhizophilus sp. nov., isolated from rhizosphere soil of mugunghwa, the national flower of South Korea. Int J Syst Evol Microbiol 2016;66:4754–4759 [CrossRef][PubMed]
    [Google Scholar]
  22. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  23. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  24. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A et al. Epibiotic Vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. Microb Ecol 2008;56:625–636 [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  27. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980;48:459–470 [CrossRef]
    [Google Scholar]
  28. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  29. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  30. Hu HY, Lim BR, Goto N, Fujie K. Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 2001;47:17–24[PubMed]
    [Google Scholar]
  31. Kroppenstedt RM. Separation of bacterial menaquinones by hplc using reverse phase (rp18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982;5:2359–2367 [CrossRef]
    [Google Scholar]
  32. Ludwig W, Strunk O, Klugbauer S, Klugbauer N, Weizenegger M et al. Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 1998;19:554–568 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003222
Loading
/content/journal/ijsem/10.1099/ijsem.0.003222
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error