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Abstract

A novel cherry-red-pigmented, Gram-stain-negative, gliding, facultatively anaerobic and rod-shaped bacterium, designated strain WTE16, was isolated from a sediment sample taken from a marine solar saltern of Wendeng, China (36° 59′ 56.49′′ N 122° 1′ 38.84′′ E). The novel isolate was able to grow at 20–40 °C (optimum 33 °C), at pH 6.0–9.0 (optimum pH 7.0) and with 1.0–12.0 % (w/v) NaCl (optimum 3.0–5.0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the most closely related validly published species is Marinilabilia salmonicolor JCM 21150 (96.0 % similarity). Average nucleotide identity, average amino acid identity, percentage of conserved proteins and digital DNA–DNA hybridization values between strain WTE16 and Marinilabilia salmonicolor JCM 21150 were 73.8 %, 73.5 %, 63.4 % and 19.5–24.2 %, respectively. The genomic DNA G+C content of strain WTE16 was 40.8 mol%. Chemotaxonomic analysis showed that the sole respiratory quinone was menaquinone 7 (MK-7), and the major fatty acids included iso-C15 : 0 and anteiso-C15 : 0. The polar lipid profile of strain WTE16 included phosphatidylethanolamine, three unidentified phospholipids and three unidentified lipids. On the basis of its phylogenetic, phenotypic, chemotaxonomic, genotypic and genomic characteristics, strain WTE16 is suggested to represent a novel species of the genus Marinilabilia , for which the name Marinilabilia rubra sp. nov. is proposed. The type strain is WTE16 (=KCTC 62599=MCCC 1H00311).

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2019-01-30
2019-10-18
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References

  1. Nakagawa Y, Yamasato K. Emendation of the genus Cytophaga and transfer of Cytophaga agarovorans and Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium-Cytophaga complex. Int J Syst Bacteriol 1996;46:599–603 [CrossRef]
    [Google Scholar]
  2. Ludwig W, Euzéby J, Whitman WB, Nakagawa Y. Road map of phylum “Bacteroidetes” and Family II. Marinilabiliaceae fam. nov. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 4 New York: Spring; 2011; pp.49–54
    [Google Scholar]
  3. Veldkamp H. A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J Gen Microbiol 1961;26:331–342 [CrossRef][PubMed]
    [Google Scholar]
  4. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of gyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. Int J Syst Bacteriol 1999;49:1551–1557 [CrossRef][PubMed]
    [Google Scholar]
  5. Shalley S, Pradip Kumar S, Srinivas TN, Suresh K, Anil Kumar P et al. Marinilabilia nitratireducens sp. nov., a lipolytic bacterium of the family Marinilabiliaceae isolated from marine solar saltern. Antonie van Leeuwenhoek 2013;103:519–525 [CrossRef][PubMed]
    [Google Scholar]
  6. Bozo-Hurtado L, García-Amado MA, Chistoserdov A, Varela R, Narvaez JJ et al. Identification of bacteria in enrichment cultures of sulfate reducers in the Cariaco Basin water column employing Denaturing Gradient Gel Electrophoresis of 16S ribosomal RNA gene fragments. Aquat Biosyst 2013;9:17–11 [CrossRef][PubMed]
    [Google Scholar]
  7. Zhou YX, Liu GH, Liu B, Chen GJ, Du ZJ. Bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern. Antonie van Leeuwenhoek 2016;109:937–943 [CrossRef]
    [Google Scholar]
  8. Sun Y, Chen BY, Du ZJ. Winogradskyella aurantia sp. nov., isolated from a marine solar saltern. Antonie van Leeuwenhoek 2017;110:1445–1452 [CrossRef][PubMed]
    [Google Scholar]
  9. Guo LY, Wang NN, Wang XQ, Chen GJ, Du ZJ et al. Lentibacillus sediminis sp. nov., isolated from a marine saltern. Int J Syst Evol Microbiol 2017;67:3946–3950 [CrossRef][PubMed]
    [Google Scholar]
  10. Wang NN, Liu ZY, Jiang LX, Li YX, Du ZJ et al. Roseovarius salinarum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2018;68:1986–1991 [CrossRef][PubMed]
    [Google Scholar]
  11. Guo LY, Ling SK, Li CM, Chen GJ, Du ZJ. Rhodosalinus sediminis gen. nov., sp. nov., isolated from marine saltern. Int J Syst Evol Microbiol 2017;67:5108–5113 [CrossRef][PubMed]
    [Google Scholar]
  12. Chen L, Wang SC, Ma CH, Zheng DX, Du ZJ et al. Ascidiaceibacter salegens gen. nov., sp. nov., isolated from an ascidian. Antonie van Leeuwenhoek 2018;111:1687–1695 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef]
    [Google Scholar]
  14. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  16. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010;20:265–272 [CrossRef]
    [Google Scholar]
  17. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  19. Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014;196:2210–2215 [CrossRef][PubMed]
    [Google Scholar]
  20. Kumar S, Subramanian S, Raghava GP, Pinnaka AK. Genome sequence of the marine bacterium Marinilabilia salmonicolor JCM 21150T. J Bacteriol 2012;194:3746 [CrossRef][PubMed]
    [Google Scholar]
  21. Stropko SJ, Pipes SE, Newman JD. Genome-based reclassification of Bacillus cibi as a later heterotypic synonym of Bacillus indicus and emended description of Bacillus indicus. Int J Syst Evol Microbiol 2014;64:3804–3809 [CrossRef][PubMed]
    [Google Scholar]
  22. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  23. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  24. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  25. Du ZJ, Wang ZJ, Zhao JX, Chen GJ. Woeseia oceani gen. nov., sp. nov., a chemoheterotrophic member of the order Chromatiales, and proposal of Woeseiaceae fam. nov. Int J Syst Evol Microbiol 2016;66:107–112 [CrossRef][PubMed]
    [Google Scholar]
  26. Kumar BV, Ramprasad EV, Sasikala C, Ramana C. Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils. Int J Syst Evol Microbiol 2013;63:200–207 [CrossRef][PubMed]
    [Google Scholar]
  27. CLSI Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement, CLSI document M100-S23. Wayne. PA: Clinical and Laboratory Standards Institute; 2013
    [Google Scholar]
  28. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 1982;5:2359–2367 [CrossRef]
    [Google Scholar]
  29. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI, Inc; 1990
    [Google Scholar]
  30. Fang D-B, Han J-R, Liu Y, Du Z-J. Seonamhaeicola marinus sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 2017;67:4857–4861 [CrossRef]
    [Google Scholar]
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