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Abstract

A Gram-stain-negative and strictly aerobic bacterium, designated DMHB-2, was isolated from a sample of seawater collected off the Yellow Sea coast of the Republic of Korea. Cells were short rods and motile by means of a single polar flagellum. Catalase and oxidase activities were positive. Growth occurred at pH 5.5–10.0 (optimum, pH 6.0), 15–45 °C (optimum, 25 °C) and with 1–9 % NaCl (optimum, 3 %). The respiratory quinone was ubiquinone-8 and the major fatty acids were C16 : 0 (17.9 %), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 26.1 %) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c; 37.4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DMHB-2 belong to the genus Marinobacterium , with the highest 16S rRNA gene sequence similarity of 95.2 % to Marinobacterium zhoushanense KCTC 42782. The genomic DNA G+C content of strain DMHB-2 was 60.8 mol%. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain DMHB-2 is suggested to represent a novel species of the genus Marinobacterium , for which the name Marinobacterium boryeongense sp. nov. is proposed. The type strain is DMHB-2 (=KACC 19225=JCM 31902).

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2018-12-19
2019-10-20
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