1887

Abstract

A novel Gram-reaction-negative bacterium, designated strain GH1-34, was isolated from a sample of tidal mudflat collected at the seashore of Gangwha Island, Republic of Korea. Cells of the bacterium were strictly aerobic, catalase- and oxidase-positive, motile by means of a polar flagellum and rod shaped. It was found to grow at 0–5 % (w/v) NaCl, 20–45 °C and pH 6–10. The major isoprenoid quinone was Q-10. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified glycolipid. The predominant fatty acids were C18 : 1ω7c and C19 : 0cyclo ω8c. The G+C content of the DNA was 70.9 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain GH1-34 formed a tight cluster with the type strain of Lutibaculum baratangense with 98.3 % sequence similarity; levels of the 16S rRNA gene sequence similarity between the novel strain and other representatives of the order ‘ Rhizobiales ’ were <95.0 %. DNA–DNA relatedness between the organism and L. baratangense KCTC 22669 was 33 %, Based on the results of phenotypic analysis and DNA–DNA hybridization experiments, strain GH1-34 (=KCTC 52847=NBRC 113277) represents a novel species of the genus Lutibaculum , for which the name Lutibaculum pontilimi sp. nov. is proposed.

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/content/journal/ijsem/10.1099/ijsem.0.003163
2018-12-12
2019-10-15
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