1887

Abstract

Bacteria isolated from lesions as well as apparently normal tissues of psittacine birds have previously been reported as taxon 37 and taxon 44 of Bisgaard. 16S rRNA gene sequence comparisons revealed a distant relationship to members of Pasteurellaceae at the species, genus and family levels. The polar lipid profile consisted of the major components phosphatidylethanolamine and phosphatidylglycerol. A new family Psittacicellaceae fam. nov. is proposed with the type genus Psittacicella gen. nov. The new genus Psittacicella includes the type species Psittacicella melopsittaci sp. nov. with type strain B96/4 (=CCUG 70858=DSM 105476), Psittacicella hinzii sp. nov. with type strain 111 (=CCUG 52861=CCM 8842) and Psittacicella gerlachiana sp. nov. with type strain EEAB3T1 (=CCUG 70857=DSM 105477). In addition to the major polar lipids, strain 111 possessed the non-identified aminophospholipids APL1 and APL2 and trace amounts of four lipids (L1–L4) whereas strain B94/4 showed the minor unidentified aminophospholipids APL3 and APL2 and trace amounts of unidentified lipid L3. These results demonstrate that strain B96/4 can be distinguished from 111 based on presence/absence of the unidentified lipids APL1 and APL3. The total polar lipid profile of strain EEAB3T1 differed from B96/4only in one minor lipid. Strain B96/4 can further be distinguished from 111 by acid formation from trehalose and raffinose and the α-glucosidase test. Strains 111 and EEAB3T1 can be separated based on acid formation from trehalose and the α-glucosidase test. Strains B96/4 and EEAB3T1 can be separated by acid formation from raffinose and eight signature indels in the RpoB protein.

Keyword(s): 16S rRNA , Amazona , budgerigar and parrot
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2018-12-13
2019-10-24
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References

  1. Bisgaard M, Hinz KH, Petersen KD, Christensen JP. Identification of members of the Pasteurellaceae isolated from birds and characterization of two new taxa isolated from psittacine birds. Avian Pathol 1999;28:369–377 [CrossRef][PubMed]
    [Google Scholar]
  2. Christensen H, Bisgaard M, Aalbaek B, Olsen JE. Reclassification of bisgaard taxon 33, with proposal of volucribacter psittacicida gen. Nov., Sp. Nov. And volucribacter amazonae sp. Nov. As new members of the pasteurellaceae. Int J Syst Evol Microbiol 2004;54:813–818 [CrossRef][PubMed]
    [Google Scholar]
  3. Gregersen RH, Neubauer C, Christensen H, Korczak B, Bojesen AM et al. Characterization of Pasteurellaceae-like bacteria isolated from clinically affected psittacine birds. J Appl Microbiol 2010;108:1235–1243 [CrossRef][PubMed]
    [Google Scholar]
  4. Bisgaard M, Xin D, Bertelsen MF, Bojesen AM, Christensen H. Prevalence of taxa of pasteurellaceae among populations of healthy captive psittacine birds. Avian Dis 2017;61:102–106 [CrossRef][PubMed]
    [Google Scholar]
  5. Christensen H, Kuhnert P, Busse HJ, Frederiksen WC, Bisgaard M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae. Int J Syst Evol Microbiol 2007;57:166–178 [CrossRef][PubMed]
    [Google Scholar]
  6. Korczak B, Christensen H, Emler S, Frey J, Kuhnert P. Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 2004;54:1393–1399 [CrossRef][PubMed]
    [Google Scholar]
  7. Korczak BM, Kuhnert P. Phylogeny of pasteurellaceae. In Kuhnert P, Christensen H. (editors) Pasteurellaceae, Biology, Genomics and Molecular Aspects Norfolk: Caister Acad. Press; 2008; pp.27–52
    [Google Scholar]
  8. Angen Ø, Ahrens P, Kuhnert P, Christensen H, Mutters R. Proposal of histophilus somni gen. Nov., Sp. Nov. For the three species incertae sedis 'haemophilus somnus', 'haemophilus agni' and 'histophilus ovis'. Int J Syst Evol Microbiol 2003;53:1449–1456 [CrossRef][PubMed]
    [Google Scholar]
  9. Christensen H, Bisgaard M, Angen O, Olsen JE. Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii. Int J Syst Evol Microbiol 2002;52:1239–1246 [CrossRef][PubMed]
    [Google Scholar]
  10. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  11. Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ et al. GenBank. Nucleic Acids Res 2017;45:D37–D42 [CrossRef][PubMed]
    [Google Scholar]
  12. Rice P, Longden I, Bleasby A. EMBOSS: the european molecular biology open software suite. Trends Genet 2000;16:276–277 [CrossRef][PubMed]
    [Google Scholar]
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  14. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  16. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W et al. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 2010;59:307–321 [CrossRef][PubMed]
    [Google Scholar]
  17. Bisgaard M, Nørskov-Lauritsen N, de Wit SJ, Hess C, Christensen H. Multilocus sequence phylogenetic analysis of Avibacterium. Microbiology 2012;158:993–1004 [CrossRef][PubMed]
    [Google Scholar]
  18. Christensen H, Kuhnert P, Nørskov-Lauritsen N, Planet PJ, Bisgaard M et al. In Stackebrandt E, Rosenberg E, Delong E, Lory S, Thompson F et al. (editors) Family Pasteurellaceae, 4th ed. New York: Springer; 2014; pp.535–564
    [Google Scholar]
  19. Stackebrandt E, Goebel BM. Taxonomic note: A place for dna-dna reassociation and 16s rrna sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  20. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  21. Auch AF, Klenk HP, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2010;2:142–148 [CrossRef][PubMed]
    [Google Scholar]
  22. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  23. Tindall BJ. A comparative study of the lipid composition of halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13:128–130 [CrossRef]
    [Google Scholar]
  24. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  25. Altenburger P, Kampfer P, Akimov VN, Lubit W, Busse H-J. Polyamine distribution in actinomycetes with group b peptidoglycan and species of the genera brevibacterium, corynebacterium, and tsukamurella. Int J Syst Bacteriol 1997;47:270–277 [CrossRef]
    [Google Scholar]
  26. Stolz A, Busse HJ, Kämpfer P. Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 2007;57:572–576 [CrossRef][PubMed]
    [Google Scholar]
  27. Mutters R, Mouahid M, Engelhard E, Mannheim W. Characterization of the family Pasteurellaceae on the basis of cellular lipids and carbohydrates. Zentralbl Bakteriol 1993;279:104–113 [CrossRef][PubMed]
    [Google Scholar]
  28. Garrity GM, Bell JA, Lilburn T. Order XIV. Pasteurellales ord. nov. In Brenner DJ, Krieg NR, Staley JT. (editors), 2nd ed.vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria) New York: Springer; 2005; pp.850
  29. Euzéby J. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Validation List No. 106. Int J Syst Evol Microbiol 2005;55:2235–2238
    [Google Scholar]
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