1887

Abstract

Moniliella yeasts were isolated from flower samples collected in Vietnam using an enrichment medium containing 50 % (w/w) glucose. The yeasts were identified as M. byzovii, M. dehoogii, M. megachiliensis, M. mellis, M. nigrescens and M. spathulata. A group of 20 strains representing a hitherto undescribed species of Moniliella was detected. ITS sequences indicated the presence of four genetic variants differing from each other by 4–14 nt. The strains, however, were identical in the TEF1 sequences and shared 1–2 nt differences in the D1/D2 regions. In the ITS–D1/D2 phylogenetic tree, the strains grouped together and formed a well-supported clade with insect-associated Moniliella species, including M. pollinis, M. megachiliensis and M. oedocephalis. The new group was most closely related to M. pollinis but differed from the latter by 95 nt (58 substitutions, 37 indels) in the ITS, 36 nt (31 substitutions, five indels) in the D1/D2, and 30 nt (30 substitutions) in the TEF1 sequences. Moniliella floricola sp. nov. is proposed to accommodate this group of isolates. The type strain and MycoBank number of M. floricola sp. nov. are TBY 30.1 (=CBS 12758=NRRL Y-63660) and MB 825274, respectively.

Keyword(s): black yeast , flowers , insect , Moniliella and Vietnam
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003099
2018-11-15
2019-10-22
Loading full text...

Full text loading...

References

  1. de Hoog GS, Smith MT, Rosa CA. Moniliella Stolk & Dakin (1966). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. London: Elsevier; 2011; pp.1837–1846
    [Google Scholar]
  2. Stolk AC, Moniliella DJC. a new genus of Moniliales. Antonie van Leeuwenhoek 1966;32:399–409
    [Google Scholar]
  3. Rosa CA, Jindamorakot S, Limtong S, Nakase T, Lachance MA et al. Synonymy of the yeast genera Moniliella and Trichosporonoides and proposal of Moniliella fonsecae sp. nov. and five new species combinations. Int J Syst Evol Microbiol 2009;59:425–429 [CrossRef][PubMed]
    [Google Scholar]
  4. Pitt J, Hocking A. Chapter 5. Primary keys and miscellaneous fungi. In Pitt J, Hocking A. (editors) Fungi and Food Spoilage London: Blackie Academic and Professional; 2009; pp.53–143
    [Google Scholar]
  5. Park JB, Yook C, Park YK. Production of erythritol by newly isolated osmophilic Trichosporon sp. Starch - Stärke 1998;50:120–123 [CrossRef]
    [Google Scholar]
  6. Thoa VK, Khanh NTM, Hien DD, Manh LD, Thanh VN et al. Screening of Moniliella yeast with high-level production of erythritol. In Proceedings of International Scientific-Practical Conference "Food, Technologies and Health" Plovdiv, Bulgaria: 2015; pp.129–134
    [Google Scholar]
  7. Rzechonek DA, Dobrowolski A, Rymowicz W, Mirończuk AM. Recent advances in biological production of erythritol. Crit Rev Biotechnol 2018;38:620–633 [CrossRef][PubMed]
    [Google Scholar]
  8. Do TTL, Thanh VN, Phan-Thi H, Cao-Hoang L, Ta TMN et al. Traditional fermented sausage “Nem chua” as a source of yeast biocatalysts efficient for the production of the aroma compound γ-decalactone. Int J Food Sci Technol 2014;49:1099–1105
    [Google Scholar]
  9. Thanh VN, Hien DD, Thom TT. Moniliella byzovii sp. nov., a chlamydospore-forming black yeast isolated from flowers. Int J Syst Evol Microbiol 2013;63:1192–1196 [CrossRef][PubMed]
    [Google Scholar]
  10. Wang QM, Theelen B, Groenewald M, Bai FY, Boekhout T. Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 2014;33:41–47 [CrossRef][PubMed]
    [Google Scholar]
  11. de Hoog GS, Guého E. Deoxyribonucleic acid base composition and taxonomy of Moniliella and allied genera. Antonie van Leeuwenhoek 1984;50:135–141 [CrossRef][PubMed]
    [Google Scholar]
  12. Thanh VN, Hien DD, Yaguchi T, Sampaio JP, Lachance MA. Moniliella sojae sp. nov., a species of black yeasts isolated from Vietnamese soy paste (tuong), and reassignment of Moniliella suaveolens strains to Moniliella pyrgileucina sp. nov., Moniliella casei sp. nov. and Moniliella macrospora emend. comb. nov. Int J Syst Evol Microbiol 2018;68:1806–1814 [CrossRef][PubMed]
    [Google Scholar]
  13. White TJ, Bruns T, Lee SJ, Taylor JL. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications 1990; pp.315–322
    [Google Scholar]
  14. O'Donnell K. Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics 1993; pp.225–233
    [Google Scholar]
  15. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005;97:84–98[PubMed]
    [Google Scholar]
  16. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013;30:772–780 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512–526 [CrossRef][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  20. Yarrow D. Chapter 11 - Methods for the isolation, maintenance and identification of yeasts. In Kurtzman CP, Fell JW. (editors) The Yeasts, 4th ed. Amsterdam: Elsevier; 1998; pp.77–100
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003099
Loading
/content/journal/ijsem/10.1099/ijsem.0.003099
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error