1887

Abstract

A Gram-stain-negative, motile, rod-shaped bacterium, designated SW127, was isolated from a seawater sample collected from the Indian Ocean. Strain SW127 was aerobic, catalase- and oxidase-positive, and grew at 8–42 °C (optimum, 30 °C), at pH 5.5–9.0 (optimum, pH 7.5) and in the presence of 0.5–11.0 % (w/v) NaCl (optimum, 3.0–4.0 %). Comparative analysis of 16S rRNA gene sequences indicated that strain SW127 belonged to the genus Alcanivorax , and closely related to Alcanivorax pacificus MCCC 1A00474 (96.7 % sequence similarity). The predominant cellular fatty acids of strain SW127 were C16 : 0 and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). Strain SW127 contained phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G+C content of strain SW127 was 62.8 mol%. On the basis of its phenotypic characteristics and phylogenetic data, strain SW127 represents a novel species of the genus Alcanivorax , for which the name Alcanivorax indicus sp. nov. is proposed. The type strain is SW127 (=CGMCC 1.16233=KCTC 62652).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003058
2018-10-11
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/12/3785.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003058&mimeType=html&fmt=ahah

References

  1. Yakimov MM, Golyshin PN, Lang S, Moore ER, Abraham WR et al. Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 1998;48:339–348 [CrossRef][PubMed]
    [Google Scholar]
  2. Fernández-Martínez J, Pujalte MJ, García-Martínez J, Mata M, Garay E et al. Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 12178T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax. Int J Syst Evol Microbiol 2003;53:331–338 [CrossRef][PubMed]
    [Google Scholar]
  3. Kyoung Kwon K, Hye Oh J, Yang SH, Seo HS, Lee JH. Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil. Int J Syst Evol Microbiol 2015;65:2204–2208 [CrossRef][PubMed]
    [Google Scholar]
  4. Yuan J, Lai Q, Wang B, Sun F, Liu X et al. Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. Int J Syst Evol Microbiol 2009;59:1158–1161 [CrossRef][PubMed]
    [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  7. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  9. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  10. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  11. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  12. Liu C, Shao Z. Isolation and characterization of oil-degrading marine micro-organisms. Acta Oceanol Sin 2005;27:114–120
    [Google Scholar]
  13. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982;16:584–586[PubMed]
    [Google Scholar]
  14. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988;38:358–361 [CrossRef]
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  16. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  17. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994; pp.121–161
    [Google Scholar]
  18. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015;25:1043–1055 [CrossRef][PubMed]
    [Google Scholar]
  19. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68:461–466 [CrossRef][PubMed]
    [Google Scholar]
  20. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  21. Liu C, Shao Z. Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 2005;55:1181–1186 [CrossRef][PubMed]
    [Google Scholar]
  22. Lai Q, Wang L, Liu Y, Fu Y, Zhong H et al. Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. Int J Syst Evol Microbiol 2011;61:1370–1374 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003058
Loading
/content/journal/ijsem/10.1099/ijsem.0.003058
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error