1887

Abstract

A novel bacterial strain, designated PR1016A, was isolated from a car air conditioning system. This rod-shaped strain showed catalase and oxidase activities, was aerobic and methylotrophic, and had a reddish pink colour. The genomic DNA G+C content of strain PR1016A was 70.2 mol%, as determined by genome sequencing. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain PR1016A was most closely related to Methylobacterium aquaticum GR16 (98.86 %), M. variabile GR3 (98.43 %), M. platani PMB 02 (98.36 %) and M. tarhaniae N4211 (98.14 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain PR1016A and M. aquaticum GR16, M. platani PMB02 and M. variabile GR3 were 88.61, 88.14 and 87.88 %, and 36.4, 35.8 and 34.7 %, respectively. Numerous insertion sequences are present in the genome of strain PR1016A, which has a larger genome than the four Methylobacterium species described above. Cells grew at 18–42 °C (optimum, 30 °C), at pH 4.0–9.0 (optimum, pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0 %). The major respiratory quinone was Q10. Fatty acid methyl ester analysis revealed that summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) was the predominant cellular fatty acid in strain PR1016A. Two-dimensional TLC indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The genotypic and phenotypic characteristics indicate that strain PR1016A represents a novel species of the genus Methylobacterium , for which the name Methylobacterium currus sp. nov. is proposed. The type strain is PR1016A (=KACC 19662=JCM 32670).

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2018-09-25
2019-10-15
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References

  1. Patt TE, Cole GC, Bland J, Hanson RS. Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy. J Bacteriol 1974;120:955–964[PubMed]
    [Google Scholar]
  2. Green PN, Bousfield IJ. Emendation of Methylobacterium (Patt, Cole, and Hanson 1976); Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig. Methylobacterium radiotolerans (Ito & Iizuka 1971) comb. nov. corrig.; and Methylobacteriu mesophilicum (Austin & Goodfellow 1979) comb. nov. Int J Syst Bacteriol 1983;33:875–877
    [Google Scholar]
  3. Green PN. The genus Methylobacterium. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H et al. (editors) The Prokaryotes, 2nd ed. New York: Springer; 1992; pp.2342–2349
    [Google Scholar]
  4. Yano T, Kubota H, Hanai J, Hitomi J, Tokuda H. Stress tolerance of Methylobacterium biofilms in bathrooms. Microbes Environ 2013;28:87–95[PubMed]
    [Google Scholar]
  5. Adams RI, Lymperopoulou DS, Misztal PK, de Cassia Pessotti R, Behie SW et al. Microbes and associated soluble and volatile chemicals on periodically wet household surfaces. Microbiome 2017;5:128 [CrossRef][PubMed]
    [Google Scholar]
  6. Kelley ST, Theisen U, Angenent LT, St Amand A, Pace NR. Molecular analysis of shower curtain biofilm microbes. Appl Environ Microbiol 2004;70:4187–4192 [CrossRef][PubMed]
    [Google Scholar]
  7. Kovaleva J, Degener JE, van der Mei HC. Methylobacterium and its role in health care-associated infection. J Clin Microbiol 2014;52:1317–1321 [CrossRef][PubMed]
    [Google Scholar]
  8. Lai CC, Cheng A, Liu WL, Tan CK, Huang YT et al. Infections caused by unusual Methylobacterium species. J Clin Microbiol 2011;49:3329–3331 [CrossRef][PubMed]
    [Google Scholar]
  9. Kressel AB, Kidd F. Pseudo-outbreak of Mycobacterium chelonae and Methylobacterium mesophilicum caused by contamination of an automated endoscopy washer. Infect Control Hosp Epidemiol 2001;22:414–418 [CrossRef][PubMed]
    [Google Scholar]
  10. Madhaiyan M, Poonguzhali S, Senthilkumar M, Lee JS, Lee KC. Methylobacterium gossipiicola sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere. Int J Syst Evol Microbiol 2012;62:162–167 [CrossRef][PubMed]
    [Google Scholar]
  11. Dourado MN, Camargo Neves AA, Santos DS, Araújo WL. Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp. Biomed Res Int 2015;2015:909016 [CrossRef][PubMed]
    [Google Scholar]
  12. Kwak MJ, Jeong H, Madhaiyan M, Lee Y, Sa TM et al. Genome information of Methylobacterium oryzae, a plant-probiotic methylotroph in the phyllosphere. PLoS One 2014;9:e106704 [CrossRef][PubMed]
    [Google Scholar]
  13. Weon HY, Kim BY, Joa JH, Son JA, Song MH et al. Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea. Int J Syst Evol Microbiol 2008;58:93–96 [CrossRef][PubMed]
    [Google Scholar]
  14. Veyisoglu A, Camas M, Tatar D, Guven K, Sazak A et al. Methylobacterium tarhaniae sp. nov., isolated from arid soil. Int J Syst Evol Microbiol 2013;63:2823–2828 [CrossRef][PubMed]
    [Google Scholar]
  15. Rice EW, Reasoner DJ, Johnson CH, Demaria LA. Monitoring for methylobacteria in water systems. J Clin Microbiol 2000;38:4296–4297[PubMed]
    [Google Scholar]
  16. Vaz-Moreira I, Nunes OC, Manaia CM. Ubiquitous and persistent Proteobacteria and other Gram-negative bacteria in drinking water. Sci Total Environ 2017;586:1141–1149 [CrossRef][PubMed]
    [Google Scholar]
  17. Bondici VF, Khan NH, Swerhone GD, Dynes JJ, Lawrence JR et al. Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings. J Appl Microbiol 2014;117:1079–1094 [CrossRef][PubMed]
    [Google Scholar]
  18. Gälli R, Leisinger T. Specialized bacterial strains for the removal of dichloromethane from industrial waste. Resour Conserv Recycl 1985;8:91–100
    [Google Scholar]
  19. Aslam Z, Lee CS, Kim KH, Im WT, Ten LN et al. Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2007;57:566–571 [CrossRef][PubMed]
    [Google Scholar]
  20. Lee Y, Jeon CO. Methylobacterium frigidaeris sp. nov., isolated from an air conditioning system. Int J Syst Evol Microbiol 2018;68:299–304 [CrossRef][PubMed]
    [Google Scholar]
  21. Kim DU, Lee H, Lee JH, Ahn JH, Lim S et al. Deinococcus metallilatus sp. nov. and Deinococcus carri sp. nov., isolated from a car air-conditioning system. Int J Syst Evol Microbiol 2015;65:3175–3182 [CrossRef][PubMed]
    [Google Scholar]
  22. Kim DU, Lee H, Kim SG, Ahn JH, Yoon Park S et al. Spirosoma aerolatum sp. nov., isolated from a motor car air conditioning system. Int J Syst Evol Microbiol 2015;65:4003–4007 [CrossRef][PubMed]
    [Google Scholar]
  23. Tani A, Ogura Y, Hayashi T, Kimbara K. Complete genome sequence of Methylobacterium aquaticum strain 22A, isolated from Racomitrium japonicum moss. Genome Announc 2015;3:3 [CrossRef][PubMed]
    [Google Scholar]
  24. Paul BJ, Ross W, Gaal T, Gourse RL. rRNA transcription in Escherichia coli. Annu Rev Genet 2004;38:749–770 [CrossRef][PubMed]
    [Google Scholar]
  25. Yona AH, Bloom-Ackermann Z, Frumkin I, Hanson-Smith V, Charpak-Amikam Y et al. tRNA genes rapidly change in evolution to meet novel translational demands. Elife 2013;2:e01339 [CrossRef][PubMed]
    [Google Scholar]
  26. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 2013;41:D590–D596 [CrossRef][PubMed]
    [Google Scholar]
  27. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680[PubMed]
    [Google Scholar]
  28. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  29. Stackebrandt E, Goebel BM. Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  30. Carroll SM, Xue KS, Marx CJ. Laboratory divergence of Methylobacterium extorquens AM1 through unintended domestication and past selection for antibiotic resistance. BMC Microbiol 2014;14:2 [CrossRef][PubMed]
    [Google Scholar]
  31. Blatt A, Bauch ME, Pörschke Y, Lohr M. A lycopene β-cyclase/lycopene ε-cyclase/light-harvesting complex-fusion protein from the green alga Ostreococcus lucimarinus can be modified to produce α-carotene and β-carotene at different ratios. Plant J 2015;82:582–595 [CrossRef][PubMed]
    [Google Scholar]
  32. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996;42:457–469
    [Google Scholar]
  33. Sasser M. Bacterial identification by gas chromatographic analysis of fatty acids methyl esters (GC-FAME). MIDI Technical Note 1990;1502:101
    [Google Scholar]
  34. Minnikina DE, O'Donnellab AG, Goodfellowb M, Aldersonb G, Athalyeb M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241
    [Google Scholar]
  35. Gallego V, García MT, Ventosa A. Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 2005;55:281–287 [CrossRef][PubMed]
    [Google Scholar]
  36. Gallego V, García MT, Ventosa A. Methylobacterium variabile sp. nov., a methylotrophic bacterium isolated from an aquatic environment. Int J Syst Evol Microbiol 2005;55:1429–1433 [CrossRef][PubMed]
    [Google Scholar]
  37. Kang YS, Kim J, Shin HD, Nam YD, Bae JW et al. Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis. Int J Syst Evol Microbiol 2007;57:2849–2853 [CrossRef][PubMed]
    [Google Scholar]
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