1887

Abstract

A Gram-stain-negative bacterium, strain BH-SD19, that was isolated from a marine sediment sample collected from the Bohai Sea, was subjected to a polyphasic taxonomic study. Cells of BH-SD19 are non-flagellated, non-gliding, oval-shaped rods, 0.5–1.0 µm wide and 1.0–2.0 µm long. BH-SD19 is strictly aerobic, and oxidase- and catalase-positive. Growth occurs at 15–40 °C (optimum 35 °C), at pH 6.0–8.5 (optimum 7.0–7.5) and with 1–10 % (w/v) NaCl (optimum 2 %). The predominant fatty acids are C19 : 0cyclo ω8c (46.5 %), C16 : 0 (20.3 %) and C18 : 1ω7c and/or C18 : 1ω6c (10.6 %). The major respiratory quinone is Q-10. The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content is 64.0 mol%. BH-SD19 shows the highest 16S rRNA sequence similarity to Pontibaca methylaminivorans (95.2 %) and strains of species of the genus Roseovarius (93.4–95.2 %). Sequence similarity values between BH-SD19 and other phylogenetically related species are all below 95.0 %. Phylogenetic trees based on 16S rRNA gene sequences indicate that BH-SD19 forms a distinct lineage and does not join any known genera in the trees. Phenotypic, chemotaxonomic and phylogenetic data indicate that BH-SD19 represents a novel genus and species in the family Rhodobacteraceae , for which the name Pelagivirga sediminicola gen. nov., sp. nov. is proposed. The type strain is BH-SD19 (=CCTCC AB 2017074=KCTC 62202).

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2018-09-17
2019-12-15
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References

  1. Garrity GM, Bell JA, Lilburn T. Order VIII. Oceanospirillales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria) New York: Springer; 2005; pp.270
    [Google Scholar]
  2. Imhoff JF, Truper HG, Pfennig N. Rearrangement of the species and genera of the phototrophic "purple nonsulfur bacteria". Int J Syst Bacteriol 1984;34:340–343 [CrossRef]
    [Google Scholar]
  3. Buchan A, González JM, Moran MA. Overview of the marine Roseobacter lineage. Appl Environ Microbiol 2005;71:5665–5677 [CrossRef][PubMed]
    [Google Scholar]
  4. Penesyan A, Breider S, Schumann P, Tindall BJ, Egan S et al. Epibacterium ulvae gen. nov., sp. nov., epibiotic bacteria isolated from the surface of a marine alga. Int J Syst Evol Microbiol 2013;63:1589–1596 [CrossRef][PubMed]
    [Google Scholar]
  5. Yoon JH, Lee SY, Jung YT, Lee JS, Lee KC. Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. Int J Syst Evol Microbiol 2013;63:2025–2031 [CrossRef][PubMed]
    [Google Scholar]
  6. Hahnke S, Tindall BJ, Schumann P, Simon M, Brinkhoff T. Pelagimonas varians gen. nov., sp. nov., isolated from the southern North Sea. Int J Syst Evol Microbiol 2013;63:835–843 [CrossRef][PubMed]
    [Google Scholar]
  7. Yin D, Chen L, Ao J, Ai C, Chen X. Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring. Int J Syst Evol Microbiol 2013;63:1868–1873 [CrossRef][PubMed]
    [Google Scholar]
  8. Lai PY, Miao L, Lee OO, Liu LL, Zhou XJ et al. Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia. Int J Syst Evol Microbiol 2013;63:1007–1012 [CrossRef][PubMed]
    [Google Scholar]
  9. Nupur, Vaidya B, Tanuku NR, Pinnaka AK. Albirhodobacter marinus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from sea shore water of Visakhapatnam, India. Antonie van Leeuwenhoek 2013;103:347–355 [CrossRef][PubMed]
    [Google Scholar]
  10. Rajasabapathy R, Mohandass C, Yoon JH, Dastager SG, Liu Q et al. Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island. Antonie van Leeuwenhoek 2015;107:589–595 [CrossRef][PubMed]
    [Google Scholar]
  11. Chen Z, Zhang J, Lei X, Lai Q, Yang L et al. Mameliella phaeodactyli sp. nov., a member of the family Rhodobacteraceae isolated from the marine algae Phaeodactylum tricornutum. Int J Syst Evol Microbiol 2015;65:1617–1621 [CrossRef][PubMed]
    [Google Scholar]
  12. Li AH, Zhou YG. Frigidibacter albus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from lake water. Int J Syst Evol Microbiol 2015;65:1199–1206 [CrossRef][PubMed]
    [Google Scholar]
  13. Li S, Tang K, Liu K, Jiao N. Thiobacimonas profunda gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from deep-sea water. Int J Syst Evol Microbiol 2015;65:359–364 [CrossRef][PubMed]
    [Google Scholar]
  14. Yang LQ, Tang L, Liu L, Salam N, Li WJ et al. Rubricella aquisinus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae. Antonie van Leeuwenhoek 2017;110:331–337 [CrossRef][PubMed]
    [Google Scholar]
  15. Chernikova TN, Dallimore J, Lünsdorf H, Heipieper HJ, Golyshin PN. Monaibacterium marinum, gen. nov, sp. nov, a new member of the Alphaproteobacteria isolated from seawater of Menai Straits, Wales, UK. Int J Syst Evol Microbiol 2017;67:3310–3317 [CrossRef][PubMed]
    [Google Scholar]
  16. Shin YH, Kim JH, Suckhoom A, Kantachote D, Kim W. Limibaculum halophilum gen. nov., sp. nov., a new member of the family Rhodobacteraceae. Int J Syst Evol Microbiol 2017;67:3812–3818 [CrossRef][PubMed]
    [Google Scholar]
  17. Li J, Huang Z, Lai Q, Liu X, Wang G et al. Oceaniglobus indicus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from surface seawater. Int J Syst Evol Microbiol 2017;67:4930–4935 [CrossRef][PubMed]
    [Google Scholar]
  18. Hou L, Sun J, Xie X, Jiao N, Zhang Y. Genome sequence of Acuticoccus yangtzensis JL1095T (DSM 28604T) isolated from the Yangtze Estuary. Stand Genomic Sci 2017;12:91 [CrossRef][PubMed]
    [Google Scholar]
  19. Dong XZ, Cai MY. Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001; pp.370–398
    [Google Scholar]
  20. Jackman SD, Vandervalk BP, Mohamadi H, Chu J, Yeo S et al. ABySS 2.0: resource-efficient assembly of large genomes using a Bloom filter. Genome Res 2017;27:768–777 [CrossRef][PubMed]
    [Google Scholar]
  21. Lane DJ. 16S/23S RNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics London: John Wiley & Sons Ltd; 1991; pp.115–175
    [Google Scholar]
  22. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  23. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  25. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  26. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  27. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988;19:161–207
    [Google Scholar]
  28. Kim KK, Lee JS, Lee KC, Oh HM, Kim SG. Pontibaca methylaminivorans gen. nov., sp. nov., a member of the family Rhodobacteraceae. Int J Syst Evol Microbiol 2010;60:2170–2175 [CrossRef][PubMed]
    [Google Scholar]
  29. Wang B, Sun F, Lai Q, Du Y, Liu X et al. Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment. Int J Syst Evol Microbiol 2010;60:1289–1295 [CrossRef][PubMed]
    [Google Scholar]
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