1887

Abstract

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea. Cells were aerobic, catalase-negative, oxidase-positive, non-motile and cocci, occurring singly, in pairs or in tetrads, and often tending to form aggregates. The strain grew at 4–45 °C (optimum, 28–37 °C), at pH 4.0–11.0 (pH 7.0–9.0) and in the presence of 0–11 % (w/v) NaCl (0–3 %). Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus . The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Whole-cell sugar analysis of strain GP-S2-8 revealed rhamnose, glucose and mannose as characteristic sugars. The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0. The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content was 74.2 mol%. DNA–DNA relatedness values between strain GP-S2-8 and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Blastococcus , for which the name Blastococcus litoris sp. nov. is proposed. The type strain is GP-S2-8 (=KCCM 43275=JCM 32354=DSM 106127=KCTC 49078).

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2018-09-14
2020-01-29
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References

  1. Ahrens R, Moll G. Ein neues knospendes Bakterium aus der OstseeA new budding bacterium from the Baltic Sea. Arch Mikrobiol 1970;70:243–265
    [Google Scholar]
  2. Urzì C, Salamone P, Schumann P, Rohde M, Stackebrandt E. Blastococcus saxobsidens sp. nov., and emended descriptions of the genus Blastococcus Ahrens and Moll 1970 and Blastococcus aggregatus Ahrens and Moll 1970. Int J Syst Evol Microbiol 2004;54:253–259 [CrossRef][PubMed]
    [Google Scholar]
  3. Lee SD. Blastococcus jejuensis sp. nov., an actinomycete from beach sediment, and emended description of the genus Blastococcus Ahrens and Moll 1970. Int J Syst Evol Microbiol 2006;56:2391–2396 [CrossRef][PubMed]
    [Google Scholar]
  4. Hezbri K, Louati M, Nouioui I, Gtari M, Rohde M et al. Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus. Int J Syst Evol Microbiol 2016;66:4864–4872 [CrossRef][PubMed]
    [Google Scholar]
  5. Zhu WY, Zhang JL, Qin YL, Xiong ZJ, Zhang DF et al. Blastococcus endophyticus sp. nov., an actinobacterium isolated from Camptotheca acuminata. Int J Syst Evol Microbiol 2013;63:3269–3273 [CrossRef][PubMed]
    [Google Scholar]
  6. Hezbri K, Nouioui I, Rohde M, Schumann P, Gtari M et al. Blastococcus colisei sp. nov, isolated from an archaeological amphitheatre. Antonie van Leeuwenhoek 2017;110:339–346 [CrossRef][PubMed]
    [Google Scholar]
  7. Hezbri K, Nouioui I, Rohde M, Spröer C, Schumann P et al. Blastococcus xanthinilyticus sp. nov., isolated from monument. Int J Syst Evol Microbiol 2018;68:1177–1183 [CrossRef][PubMed]
    [Google Scholar]
  8. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  9. Lee DW, Lee SD. Aeromicrobium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2008;58:987–991 [CrossRef][PubMed]
    [Google Scholar]
  10. Lee DW, Park MY, Kim JJ, Kim BS. Pseudokineococcus basanitobsidens sp. nov., isolated from volcanic rock. Int J Syst Evol Microbiol 2017;67:3824–3828 [CrossRef][PubMed]
    [Google Scholar]
  11. Lane D. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester, UK: John Wiley and Sons; 1991; pp.115–175
    [Google Scholar]
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  16. Jukes T, Cantor C. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969; pp.21–132
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  18. Komagata K, Suzuki K-I. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207
    [Google Scholar]
  19. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  20. Uchida K, Aida KO. An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 1984;30:131–134 [CrossRef]
    [Google Scholar]
  21. Kroppenstedt R. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985; pp.173–199
    [Google Scholar]
  22. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  23. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the g+c content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  24. Normand P. Geodermatophilaceae fam. nov., a formal description. Int J Syst Evol Microbiol 2006;56:2277–2278 [CrossRef][PubMed]
    [Google Scholar]
  25. Normand P, Daffonchio D, Gtari M. The family Geodermatophilaceae. The Prokaryotes Berlin Heidelberg: Springer; 2014; pp.361–379
    [Google Scholar]
  26. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  27. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  28. Hirayama H, Tamaoka J, Horikoshi K. Improved immobilization of DNA to microwell plates for DNA-DNA hybridization. Nucleic Acids Res 1996;24:4098–4099 [CrossRef][PubMed]
    [Google Scholar]
  29. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
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