1887

Abstract

A halophilic archaeon, strain MBLA0036, was isolated from Sorae solar saltern near Incheon, Republic of Korea. Strain MBLA0036 had three 16S rRNA genes: rrnA, rrnB and rrnC. The 16S rRNA gene sequence similarities between strain MBLA0036 (based on the rrnA gene) and Haloplanus ruber R35 and Haloplanus litoreus GX21 were 98.0 and 97.3 %, respectively. The similarities of the RNA polymerase subunit B′ gene between strain MBLA0036 and H. ruber R35 and H. litoreus GX21 were 94.0 and 92.1 %, respectively. Cells of strain MBLA0036 were Gram-stain-negative, motile, red-pigmented, pleomorphic, flat and contained gas vesicles. Strain MBLA0036 grew at 15‒55 °C (optimum, 37 °C), in 10‒30 % (w/v) NaCl (15 %, w/v) with 0‒0.5 M MgSO4.7H2O (0.2 M) and at pH 6.0–9.0 (pH 7.0). The cells lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % (w/v). Major polar lipids of strain MBLA0036 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a glycolipid that was chromatographically identical to sulfated mannosyl glucosyl diether. The major isoprenoid quinone was menaquinone-8. The genomic DNA G+C content was 65.5 mol%. DNA–DNA hybridization values between strain MBLA0036 and H. ruber JCM 17271 and H. litoreus JCM 17092 were 35±3 and 18±1 %, respectively. Therefore, strain MBLA0036 is described a novel species of the Haloplanus , for which we propose the name Haloplanus rallus sp. nov. The type strain is MBLA0036 (=KCTC 4239=JCM 31425).

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2018-08-21
2019-12-15
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