Kimura and co-workers (Kimura N et al. Int J Syst Evol Microbiol 2018;68:1429–1435) recently proposed renaming the obligate aerobe Pseudomonas pseudoalcaligenes KF707 as Pseudomonas furukawiisp. nov. type strain KF707. Since the first quasi-complete genome sequence of KF707 was reported in 2012 (accession number: PRJNA83639) numerous reports on chemotaxis and function/composition of the respiratory redox chain of KF707 have been published, demonstrating that KF707 contains three cheA genes for aerobic motility, four cytochrome oxidases of c(c)aa 3- and cbb 3-type and one bd-type quinol oxidase. With this background in mind, it has been quite a surprise to read within Table 1 of the paper by Kimura et al. that strain KF707 is phenotypically characterized as cytochrome oxidase-negative. Further, Table 1 also reports that KF707 is β-galactosidase-positive, an affirmation that is not consistent with results documented in the current literature. In this present ‘Letter to the Editor’ we show that Kimura et al. have contradicted themselves and provided inaccurate information in respect to the respiratory phenotypic features of P. furukawii. Based on this, an official corrigendum is requested since the publication, as it is, blurs the credibility of the International Journal of Systematic and Evolutionary Microbiology.


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  1. Kimura N, Watanabe T, Suenaga H, Fujihara H, Futagami T et al. Pseudomonas furukawii sp. nov., a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil in Japan. Int J Syst Evol Microbiol 2018; 68:1429–1435 [View Article][PubMed]
    [Google Scholar]
  2. Furukawa K, Miyazaki T. Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. J Bacteriol 1986; 166:392–398 [View Article][PubMed]
    [Google Scholar]
  3. Fedi S, Carnevali M, Fava F, Andracchio A, Zappoli S et al. Polychlorinated biphenyl degradation activities and hybridization analyses of fifteen aerobic strains isolated from a PCB-contaminated site. Res Microbiol 2001; 152:583–592 [View Article][PubMed]
    [Google Scholar]
  4. Tremaroli V, Fedi S, Turner RJ, Ceri H, Zannoni D. Pseudomonas pseudoalcaligenes KF707 upon biofilm formation on a polystyrene surface acquire a strong antibiotic resistance with minor changes in their tolerance to metal cations and metalloid oxyanions. Arch Microbiol 2008; 190:29–39 [View Article][PubMed]
    [Google Scholar]
  5. Tremaroli V, Vacchi-Suzzi C, Fedi S, Ceri H, Zannoni D et al. Tolerance of Pseudomonas pseudoalcaligenes KF707 to metals, polychlorobiphenyls and chlorobenzoates: effects on chemotaxis-, biofilm- and planktonic-grown cells. FEMS Microbiol Ecol 2010; 74:291–301 [View Article][PubMed]
    [Google Scholar]
  6. Booth SC, George IF, Zannoni D, Cappelletti M, Duggan GE et al. Effect of aluminium and copper on biofilm development of Pseudomonas pseudoalcaligenes KF707 and P. fluorescens as a function of different media compositions. Metallomics 2013; 5:723–735 [View Article][PubMed]
    [Google Scholar]
  7. Booth SC, Weljie AM, Turner RJ. Metabolomics reveals differences of metal toxicity in cultures of Pseudomonas pseudoalcaligenes KF707 grown on different carbon sources. Front Microbiol 2015; 6:827 [View Article][PubMed]
    [Google Scholar]
  8. Furukawa K, Hirose J, Suyama A, Zaiki T, Hayashida S. Gene components responsible for discrete substrate specificity in the metabolism of biphenyl (bph operon) and toluene (tod operon). J Bacteriol 1993; 175:5224–5232 [View Article][PubMed]
    [Google Scholar]
  9. Di Tomaso G, Fedi S, Carnevali M, Manegatti M, Taddei C et al. The membrane-bound respiratory chain of Pseudomonas pseudoalcaligenes KF707 cells grown in the presence or absence of potassium tellurite. Microbiology 2002; 148:1699–1708 [View Article][PubMed]
    [Google Scholar]
  10. Triscari-Barberi T, Simone D, Calabrese FM, Attimonelli M, Hahn KR et al. Genome sequence of the polychlorinated-biphenyl degrader Pseudomonas pseudoalcaligenes KF707. J Bacteriol 2012; 194:4426–4427 [View Article][PubMed]
    [Google Scholar]
  11. Fedi S, Barberi TT, Nappi MR, Sandri F, Booth S et al. The role of cheA genes in swarming and swimming motility of Pseudomonas pseudoalcaligenes KF707. Microbes Environ 2016; 31:169–172 [View Article][PubMed]
    [Google Scholar]
  12. Sandri F, Fedi S, Cappelletti M, Calabrese FM, Turner RJ et al. Biphenyl modulates the expression and function of respiratory oxidases in the polychlorinated-biphenyls degrader Pseudomonas pseudoalcaligenes KF707. Front Microbiol 2017; 8:1223 [View Article][PubMed]
    [Google Scholar]
  13. Sandri F, Musiani F, Selamoglu N, Daldal F, Zannoni D. Pseudomonas pseudoalcaligenes KF707 grown with biphenyl expresses a cytochrome caa3 oxidase that uses cytochrome c4 as electron donor. FEBS Lett 2018; 592:901–915 [View Article][PubMed]
    [Google Scholar]


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