1887

Abstract

A strictly aerobic, catalase-negative and oxidase-positive bacterium (HR-BB), isolated from a water sample of the Han River, was taxonomically studied using a polyphasic approach. Cells were Gram-stain-negative motile rods with a polar flagellum. The strain grew at 20–35 °C and pH 7–8 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-BB belonged to the family Nevskiaceae in the phylum Proteobacteria and formed a phylogenic lineage with members of the genus Solimonas . A comparison of the 16S rRNA gene sequences of strain HR-BB and the type strains of closely related species of the genus Solimonas showed that it shared highest sequence similarity with Solimonas terrae KIS83-12 (94.9 %), Solimonas soli DCY12 (94.8 %), Solimonas variicoloris MN28 (94.4 %) and Solimonas flava CW-KD 4 (94.2 %). The fatty acids of the strain consisted of summed features 8 (comprising C18 : 1 ω6c and/or C18 : 1 ω7c) and 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), C16 : 0 and C12 : 0 as major components. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids and an unidentified lipid. Ubiquinone-8 was detected as the sole respiratory quinone. The DNA G+C content of strain HR-BB was 68.5 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-BB represents a novel species of the genus Solimonas , for which the name Solimonas fluminis sp. nov. is proposed. The type strain is HR-BB (=KACC 19410=JCM 32268).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002865
2018-07-17
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/9/2755.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002865&mimeType=html&fmt=ahah

References

  1. Kim MK, Kim YJ, Cho DH, Yi TH, Soung NK et al. Solimonas soli gen. nov., sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2007;57:2591–2594 [CrossRef][PubMed]
    [Google Scholar]
  2. Naushad S, Adeolu M, Wong S, Sohail M, Schellhorn HE et al. A phylogenomic and molecular marker based taxonomic framework for the order Xanthomonadales: proposal to transfer the families Algiphilaceae and Solimonadaceae to the order Nevskiales ord. nov. and to create a new family within the order Xanthomonadales, the family Rhodanobacteraceae fam. nov., containing the genus Rhodanobacter and its closest relatives. Antonie van Leeuwenhoek 2015;107:467–485 [CrossRef][PubMed]
    [Google Scholar]
  3. Sheu SY, Cho NT, Arun AB, Chen WM. Proposal of Solimonas aquatica sp. nov., reclassification of Sinobacter flavus Zhou et al. 2008 as Solimonas flava comb. nov. and Singularimonas variicoloris Friedrich and Lipski 2008 as Solimonas variicoloris comb. nov. and emended descriptions of the genus Solimonas and its type species Solimonas soli. Int J Syst Evol Microbiol 2011;61:2284–2291 [CrossRef][PubMed]
    [Google Scholar]
  4. Zhou Y, Zhang YQ, Zhi XY, Wang X, Dong J et al. Description of Sinobacter flavus gen. nov., sp. nov., and proposal of Sinobacteraceae fam. nov. Int J Syst Evol Microbiol 2008;58:184–189 [CrossRef][PubMed]
    [Google Scholar]
  5. Friedrich MM, Lipski A. Alkanibacter difficilis gen. nov., sp. nov. and Singularimonas variicoloris gen. nov., sp. nov., hexane-degrading bacteria isolated from a hexane-treated biofilter. Int J Syst Evol Microbiol 2008;58:2324–2329 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim SJ, Moon JY, Weon HY, Ahn JH, Chen WM et al. Solimonas terrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 2014;64:1218–1222 [CrossRef][PubMed]
    [Google Scholar]
  7. Lee Y, Jeon CO. Sphingomonas frigidaeris sp. nov., isolated from an air conditioning system. Int J Syst Evol Microbiol 2017;67:3907–3912 [CrossRef][PubMed]
    [Google Scholar]
  8. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  9. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007;3:e56 [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J. PHYLIP (Phylogeny Inference Package), Version 3.6a Seattle: Department of genetics, University of Washington, Seattle, WA, USA; 2002
    [Google Scholar]
  11. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014;30:1312–1313 [CrossRef][PubMed]
    [Google Scholar]
  12. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  13. Rosselló-Móra R, Amann R. Past and future species definitions for Bacteria and Archaea. Syst Appl Microbiol 2015;38:209–216 [CrossRef][PubMed]
    [Google Scholar]
  14. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  15. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1988;19:1–67
    [Google Scholar]
  16. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  17. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988;19:161–208
    [Google Scholar]
  18. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  19. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977;27:104–117 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002865
Loading
/content/journal/ijsem/10.1099/ijsem.0.002865
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error