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Abstract

A bacterial strain, designated 17Sr1-1, was isolated from gamma ray-irradiated soil. Cells of this strain were Gram-stain-negative, strictly aerobic, motile and non-spore-forming rods. Growth occurred at 18–42 ˚C and pH 6.0–8.0, but no growth occurred at 2 % NaCl concentration. The major fatty acids of strain 17Sr1-1 were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), iso-C17 : 1 ω5c and C16 : 0. The polar lipid profile contained diphosphatidylglycerol, glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified lipids. The G+C content of the genomic DNA of 17Sr1-1 was 71.9 mol%. The 16S rRNA gene sequence analysis showed that strain 17Sr1-1 was phylogenetically related to Roseomonas pecuniae N75 and Roseomonas rosea 173-96 (96.6 and 96.3 % sequence similarity, respectively). The genotypic and phenotypic data showed that strain 17Sr1-1 could be distinguished from its phylogenetically related species, and that this strain represented a novel species within the genus Roseomonas , for which the name Roseomonas radiodurans sp. nov. (type strain 17Sr1-1=KCTC 52899=NBRC 112872) is proposed as the first reported gamma ray-resistant Roseomonas species.

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2018-06-19
2020-01-22
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References

  1. Rihs JD, Brenner DJ, Weaver RE, Steigerwalt AG, Hollis DG et al. Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 1993;31:3275–3283[PubMed]
    [Google Scholar]
  2. Lopes A, Santo CE, Grass G, Chung AP, Morais PV. Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin. Int J Syst Evol Microbiol 2011;61:610–615 [CrossRef][PubMed]
    [Google Scholar]
  3. Yoo SH, Weon HY, Noh HJ, Hong SB, Lee CM et al. Roseomonas aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 2008;58:1482–1485 [CrossRef][PubMed]
    [Google Scholar]
  4. Srinivasan S, Lee S-Y, Kim MK, Jung H-Y. Complete genome sequence of Hymenobacter sp. DG25A, a gamma radiation-resistant bacterium isolated from soil. Mol Cell Toxicol 2017;13:65–72 [CrossRef]
    [Google Scholar]
  5. Skerman VBD. A Guide to the Identification of the Genera of Bacteria, 2nd ed. Baltimore: Williams & Wilkins; 1967
    [Google Scholar]
  6. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murra RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  7. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  8. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  9. Embley TM, Wait R. Structurallipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994; pp.121–161
    [Google Scholar]
  10. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207
    [Google Scholar]
  11. Collins MD, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 1981;45:316–354[PubMed]
    [Google Scholar]
  12. Shin YK, Lee JS, Chun CO, Kim HJ, Park YH. Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 1996;6:68–69
    [Google Scholar]
  13. Kim MK, Kang MS, Srinivasan S, Lee DH, Lee SY et al. Complete genome sequence of Hymenobacter sedentarius DG5BT, a bacterium resistant to gamma radiation. Mol Cell Toxicol 2017;13:199–205 [CrossRef]
    [Google Scholar]
  14. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  15. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  16. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012;28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  19. Fitch WM. Toward defining course of evolution – minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  21. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  22. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773 [CrossRef][PubMed]
    [Google Scholar]
  23. Sánchez-Porro C, Gallego V, Busse HJ, Kämpfer P, Ventosa A. Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas. Int J Syst Evol Microbiol 2009;59:1193–1198 [CrossRef][PubMed]
    [Google Scholar]
  24. Sl Y, Lee SK. Ultraviolet radiation: DNA damage, repair, and human disorders. Mol Cell Toxicol 2017;13:21–28
    [Google Scholar]
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