1887

Abstract

A Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacterium, designated strain RPE64, was isolated from the gut symbiotic organ of the bean bug , collected in Tsukuba, Japan, in 2007. 16S rRNA gene sequencing showed that this strain belongs to the clade, exhibiting the highest sequence similarity to LMG 29314 (100 %), LMG 29316 (99.52 %) and LMG 29326 (99.04 %). Phylogenomic analyses based on 107 single-copy core genes and Genome Distance Phylogeny confirmed LMG 29314 LMG 29326 and several uncultivated, endophytic species as its nearest phylogenetic neighbours. Digital DNA–DNA hybridization experiments unambiguously demonstrated that strain RPE64 represents a novel species in this lineage. The G+C content of its genome was 63.2 mol%. The isoprenoid quinone was ubiquinone 8 and the predominant fatty acid components were C, C 7 and C cyclo. The absence of nitrate reduction and the capacity to grow at pH 8 clearly differentiated strain RPE64 from related species. Based on these genotypic and phenotypic characteristics, strain RPE64 is classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RPE64 (=NCIMB 15023=JCM 31142).

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2018-07-01
2019-12-13
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