1887

Abstract

Three strains (DMKU-XE11, DMKU-XE15 and DMKU-XE20) representing a single novel anamorphic and -xylose-fermenting yeast species were obtained from three peat samples collected from Khan Thulee peat swamp forest in Surat Thani province, Thailand. The strains differed from each other by one to two nucleotide substitutions in the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and zero to one nucleotide substitution in the internal transcribed spacer (ITS) region. Phylogenetic analysis based on the combined sequences of the ITS and the D1/D2 regions showed that the three strains represented a single species that was distinct from the other related species in the / clade. The three strains form a subclade with the other species including , and was the most closely related species, with 2.1–2.4 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 3.8–4.0 % nucleotide substitutions in the ITS region. The three strains (DMKU-XE11, DMKU-XE15 and DMKU-XE20) were assigned as a single novel species, which was named sp. nov. The type strain is DMKU-XE11 (=CBS 15219=TBRC 7764). The MycoBank number for sp. nov. is MB 824179.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002835
2018-07-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/7/2313.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002835&mimeType=html&fmt=ahah

References

  1. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Boekhout, (1923). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.987–1278
    [Google Scholar]
  2. Daniel HM, Lachance MA, Kurtzman CP. On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie van Leeuwenhoek 2014;106:67–84 [CrossRef][PubMed]
    [Google Scholar]
  3. Kurtzman CP. Lodderomyces van der Walt (1971). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.561–563
    [Google Scholar]
  4. Kurtzman CP, Suzuki M. Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience 2010;51:2–14 [CrossRef]
    [Google Scholar]
  5. Urbina H, Blackwell M. Multilocus phylogenetic study of the Scheffersomyces yeast clade and characterization of the N-terminal region of xylose reductase gene. PLoS One 2012;7:e39128 [CrossRef][PubMed]
    [Google Scholar]
  6. Nguyen NH, Suh SO, Blackwell M. Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia 2007;99:842–858 [CrossRef][PubMed]
    [Google Scholar]
  7. Suh SO, Nguyen NH, Blackwell M. Yeasts isolated from plant-associated beetles and other insects: seven novel Candida species near Candida albicans. FEMS Yeast Res 2008;8:88–102 [CrossRef][PubMed]
    [Google Scholar]
  8. Kurtzman CP. Scheffersomyces Kurtzman & M. Suzuki (2010). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.773–777
    [Google Scholar]
  9. Nguyen NH, Suh SO, Blackwell M. Spathaspora N.H. Nguyen, S.-O. Suh & M. Blackwell (2006). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.795–797
    [Google Scholar]
  10. Nguyen NH, Suh SO, Marshall CJ, Blackwell M. Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res 2006;110:1232–1241 [CrossRef][PubMed]
    [Google Scholar]
  11. Cadete RM, Santos RO, Melo MA, Mouro A, Gonçalves DL et al. Spathaspora arborariae sp. nov., a D-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 2009;9:1338–1342 [CrossRef][PubMed]
    [Google Scholar]
  12. Agbogbo FK, Wenger KS. Production of ethanol from corn stover hemicellulose hydrolyzate using Pichia stipitis. J Ind Microbiol Biotechnol 2007;34:723–727 [CrossRef][PubMed]
    [Google Scholar]
  13. Suh SO, Marshall CJ, McHugh JV, Blackwell M. Wood ingestion by passalid beetles in the presence of xylose-fermenting gut yeasts. Mol Ecol 2003;12:3137–3145 [CrossRef][PubMed]
    [Google Scholar]
  14. Urbina H, Frank R, Blackwell M. Scheffersomyces cryptocercus: a new xylose-fermenting yeast associated with the gut of wood roaches and new combinations in the Sugiyamaella yeast clade. Mycologia 2013;105:650–660 [CrossRef][PubMed]
    [Google Scholar]
  15. Suh SO, Houseknecht JL, Gujjari P, Zhou JJ. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov. and Candida manassasensis sp. nov., novel yeasts associated with wood-ingesting insects, and their ecological and biofuel implications. Int J Syst Evol Microbiol 2013;63:4330–4339 [CrossRef][PubMed]
    [Google Scholar]
  16. Jindamorakot S, Limtong S, Yongmanitchai W, Tuntirungkij M, Potacharoen W et al. Two new anamorphic yeasts, Candida thailandica sp. nov. and Candida lignicola sp. nov., isolated from insect frass in Thailand. FEMS Yeast Res 2007;7:1409–1414 [CrossRef][PubMed]
    [Google Scholar]
  17. Cadete RM, Melo MA, Dussán KJ, Rodrigues RC, Silva SS et al. Diversity and physiological characterization of d-xylose-fermenting yeasts isolated from the Brazilian Amazonian Forest. PLoS One 2012;7:e43135 [CrossRef][PubMed]
    [Google Scholar]
  18. Ramírez C, González A. Five new filamentous, glucose-fermenting Candida isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. Mycopathologia 1984;88:83–92 [CrossRef]
    [Google Scholar]
  19. Morais CG, Cadete RM, Uetanabaro AP, Rosa LH, Lachance MA et al. d-xylose-fermenting and xylanase-producing yeast species from rotting wood of two Atlantic Rainforest habitats in Brazil. Fungal Genet Biol 2013;60:19–28 [CrossRef][PubMed]
    [Google Scholar]
  20. Polburee P, Lertwattanasakul N, Limtong P, Groenewald M, Limtong S. Nakazawaea todaengensis f.a., sp. nov., a yeast isolated from a peat swamp forest in Thailand. Int J Syst Evol Microbiol 2017;67:2377–2382 [CrossRef][PubMed]
    [Google Scholar]
  21. Jaiboon K, Lertwattanasakul N, Limtong P, Limtong S. Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes. Mycol Prog 2016;15:755–770 [CrossRef]
    [Google Scholar]
  22. Thormann MN, Rice AV, Beilman DW. Yeasts in peatlands: a review of richness and roles in peat decomposition. Wetlands 2007;27:761–773 [CrossRef]
    [Google Scholar]
  23. Kachalkin AV, Yurkov AM. Yeast communities in Sphagnum phyllosphere along the temperature-moisture ecocline in the boreal forest-swamp ecosystem and description of Candida sphagnicola sp. nov. Antonie van Leeuwenhoek 2012;102:29–43 [CrossRef][PubMed]
    [Google Scholar]
  24. Grum-Grzhimaylo OA, Debets AJ, Bilanenko EN. The diversity of microfungi in peatlands originated from the White Sea. Mycologia 2016;108:233–254 [CrossRef][PubMed]
    [Google Scholar]
  25. Limtong S, Yongmanitchai W, Kawasaki H, Seki T. Candida thaimueangensis sp. nov., an anamorphic yeast species from estuarine water in a mangrove forest in Thailand. Int J Syst Evol Microbiol 2007;57:650–653 [CrossRef][PubMed]
    [Google Scholar]
  26. O’Donnell K. Fusarium and its near relatives. In Reynolds DR, Taylor JW. (editors) The Fungal Holomorph: Mitotic and Pleomorphic Speciation in Fungal Systematics Wallingford, UK: CAB International; 1993; pp.225–233
    [Google Scholar]
  27. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990; pp.315–322
    [Google Scholar]
  28. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  29. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  30. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  31. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  32. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.87–110
    [Google Scholar]
  33. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  34. Kurtzman CP, Fell JW, Boekhout T. Gene sequence analyses and other DNA-based methods for yeast species recognition. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.137–144
    [Google Scholar]
  35. Hui FL, Niu QH, Ke T, Li YX, Lee CF. Candida sanyaensis sp. nov., an ascomycetous yeast species isolated from soil. Antonie van Leeuwenhoek 2013;103:47–52 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002835
Loading
/content/journal/ijsem/10.1099/ijsem.0.002835
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error