1887

Abstract

Strain GI5 was isolated from a surface seawater sample collected from Garorim Bay (West Sea, Republic of Korea). The isolated strain was aerobic, Gram-stain-negative, rod-shaped, motile by means of a polar flagellum, negative for catalase and weakly positive for oxidase. The optimum growth pH, salinity and temperature were determined to be pH 7.5–8.0, 3 % NaCl (w/v) and 25 °C, respectively; the growth ranges were pH 6.0–9.0, 1–7 % NaCl (w/v) and 18–40 °C. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that GI5 clustered within the family Alcanivoracaceae , and most closely with Alcanivorax dieselolei B-5 and Alcanivorax marinus R8-12 (91.9 % and 91.6 % similarity, respectively). The major cellular fatty acids in GI5 were C18 : 1ω7c/C18 : 1ω6c (44.45 %), C16 : 1ω6c/C16 : 1ω7c (14.17 %) and C16 : 0 (10.19 %); this profile was distinct from those of the closely related species. The major respiratory quinone of GI5 was Q-8. The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Two putative alkane hydroxylase (alkB) genes were identified in GI5. The G+C content of the genomic DNA of GI5 was determined to be 51.2 mol%. On the basis of the results of phenotypic, chemotaxonomic and phylogenetic studies, strain GI5 represents a novel species of a novel genus of the family Alcanivoracaceae, for which we propose the name Ketobacter alkanivorans gen. nov., sp. nov.; the type strain is GI5 (=KCTC 52659=JCM 31835).

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2018-05-29
2019-10-23
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