1887

Abstract

A Gram-stain negative, alkaliphilic and halotolerant bacterium, designated CCL18, was isolated from Lake Cuochuolong on the Tibetan Plateau. The strain was aerobic, short rod-shaped, catalase- and oxidase-positive, and motile by means of several polar flagella. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain CCL18 belongs to the genus , with its two closest neighbours being B2 (96.6 %, 16S rRNA gene sequence similarity) and 1_C16_27 (96.1 %). The predominant respiratory quinone of strain CCL18 was Q-10, with Q-9 as a minor component. The major fatty acids were Cω6/Cω7 (60.4 %), Ccyclo ω8 (8.1 %) and C (6.8 %). The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, seven kinds of unidentified lipids and three kinds of glycolipids. The DNA G+C content was 60.1 mol%. DNA−DNA hybridization showed 35.2 % relatedness between strain CCL18 and B2 and 24.6 % relatedness to 1_C16_27. Based on phylogenetic analysis, DNA−DNA hybridization and a range of physiological and biochemical characteristics, strain CCL18 was clearly distinguishable from the other strains of the genus . It was evident that strain CCL18 could be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CCL18 (=CGMCC 1.16231=KCTC 62030).

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2018-07-01
2020-01-29
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